在人体各组织中,脑的结构与功能最为复杂。即使是一个最简单的动作,常涉及到许多神经元及其突触的活动。为了进一步研究脑内各种功能及其活动的机理,体外培养神经元是一个较好的研究方法。近年来,国外不少学者,如克雷恩(Crain)和尼尔(Neale)等,应用辣根过氧化物酶(horse radish peroxidase,简称HRP)作培养神经元的逆行传递,或用细胞内注射HRP,来显示各种神经元及其相互间的联接。他们还结合细胞电生理的研究,用玻璃微电极记录培养的脊髓神经元的电反应。细胞内注射HRP在光镜和电镜下可清晰地显示原代分离单层细胞培养的小鼠脊 In human tissues, the structure and function of the brain are the most complex. Even the simplest of actions often involves the activity of many neurons and their synapses. In order to further study the various functions of the brain and its activity mechanism, in vitro cultured neurons is a better research method. In recent years, many foreign scholars, such as Crain and Neale, applied horseradish peroxidase (HRP) as retrograde neurons in culture, HRP injection to show various neurons and their connections. They also combined with electrophysiological studies of cells using glass microelectrodes to record the electrical responses of cultured spinal cord neurons. Intracellular injection of HRP clearly shows, under light and electron microscopy, mouse spines cultured in primary isolated monolayers