论文部分内容阅读
本研究利用农杆菌介导法获得的过表达At CBF4基因、过表达Bo WS基因大豆株系的后代,研究其在干旱胁迫下转基因大豆生理特性、光合性状以及产量性状的表现,以揭示其转基因大豆耐旱性特点。研究结果表明:过表达CBF4基因株系在丙二醛、气孔导度、细胞CO2浓度、蒸腾速率,单株荚数、单株粒数上表现优于过表达Bo WS基因大豆株系;过表达Bo WS基因大豆株系在脯氨酸、可溶性糖、净光合速率、瞬时水分利用率、株高、节数、百粒重、单株产量以及结荚期单株荚数和单株粒数上耐旱性更强。研究结果表明通过灰色关联分析以及聚类分析表明过表达Bo WS基因大豆株系比过表达At CBF4基因株系具有更强的耐旱性。
In this study, the offspring of overexpressing At CBF4 gene overexpressing Bo WS gene were obtained by Agrobacterium tumefaciens-mediated method to study the physiological, photosynthetic and photosynthetic characteristics of transgenic soybean under drought stress to reveal its transgenic Drought tolerance of soybean. The results showed that overexpression of CBF4 was superior to Bo WS gene overexpressing soybean lines in the aspects of malondialdehyde, stomatal conductance, CO2 concentration, transpiration rate, number of pods per plant, Proline, soluble sugar, net photosynthetic rate, instantaneous water use efficiency, plant height, number of stalks, 100-grain weight per plant, yield per plant, pod number per plant and grain number per plant in Bo WS gene Drought tolerance stronger. The results showed that the soybean plants overexpressing the Bo WS gene have stronger drought tolerance than the At CBF4 overexpression strains by gray relational analysis and cluster analysis.