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为了阐明大豆受尖孢镰刀菌(Fusarium oxysporum,Fo)侵染后在基因转录水平上的感病反应,本研究用cDNA-AFLP方法,对Fo浸根接种后大豆根组织进行了基因转录谱的银染法检测分析。结果表明,在测定的大约1 000个大豆cDNA扩增片段(TDFs)中,16个是差异性表达的,其中9个受Fo诱导上调表达,7个被抑制下调表达。对这些差异性表达的TDFs进行克隆、测序及其同源性进行分析,发现其中6个具有已知或推断的生物学功能,包括钙调素结合蛋白(CaMBP)、BURP结构域蛋白和羟基多花碱-羟基烷宁转移酶等,其余10个功能未知。这些可能参与大豆感病性的差异性表达新基因片段的发现,为开展大豆感病性功能基因组学研究提供了材料。
In order to elucidate the susceptibility of soybean to gene transcription at the level of Fusarium oxysporum (Fo) infection, the cDNA-AFLP method was used to study the gene transcriptional profile of soybean root after inoculation with Fo Silver staining test analysis. The results showed that among the about 1000 soybean cDNA amplification fragments (TDFs), 16 were differentially expressed, of which 9 were upregulated by Fo and 7 were downregulated. Of these differentially expressed TDFs, cloned, sequenced, and their homologies were analyzed and found that six of them had known or inferred biological functions including calmodulin-binding protein (CaMBP), BURP domain protein and hydroxyl poly Spermine hydroxyalkyltransferase, the remaining 10 functions unknown. These findings, which may be involved in the differential expression of novel gene segments in soybean susceptibility, provide the material for carrying out functional genomics research on soybean susceptibility.