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目的:观察运动对大鼠心肌、骨骼肌硫氧还蛋白还原酶(TR)的影响。方法:SD大鼠30只,随机分成安静对照组、耐力训练组和力竭组。训练组进行6周渐增游泳训练,5次/周。最后1次训练后2 4小时,断头处死安静对照组和训练组大鼠,力竭组大鼠在一次性力竭运动后即刻处死。DTNB法检测心肌、骨骼肌TR活性,RT -PCR法观察TRmRNA水平变化。结果:6周耐力训练大鼠心肌TR活性显著高于安静对照组,一次性力竭组大鼠心肌TR活性显著低于安静对照组。各组大鼠心肌TRmRNA均无显著性差异。结论:6周耐力训练显著提高大鼠心肌TR活性,而在mRNA水平上无显著性差异。
Objective: To observe the effect of exercise on the thioredoxin reductase (TR) in myocardium and skeletal muscle of rats. Methods: Thirty SD rats were randomly divided into quiet control group, endurance training group and exhaustive group. Training group 6 weeks of incremental swimming training, 5 times / week. At 24 hours after the last training, the rats in the control and training groups were sacrificed and the rats in the exhausted group were sacrificed immediately after a one-time exhaustive exercise. TRNB activity of cardiac muscle and skeletal muscle was detected by DTNB method, and TR mRNA level was observed by RT-PCR. Results: The myocardial TR activity in 6-week endurance-trained rats was significantly higher than that in the quiet control rats. The TR activity in the rats in the once-exhausted rats group was significantly lower than that in the quiet control rats. There was no significant difference in myocardial TRmRNA in all groups. Conclusion: 6-week endurance training significantly increased TR activity in rat myocardium, but no significant difference in mRNA level.