“共价组装”类反应型分子探针的核心特点是待测底物能引发两个分子片段之间的反应,进而在检测体系原位组装出染料的推拉电子共轭链,并产生一个高灵敏的信号.在本课题组提出“共价组装”的概念之前,已有多种此类探针被报道.本文对不同时期、不同课题组的具有“共价组装”特点的典型探针和检测试剂进行了总结. The key feature of the covalent assembly type molecular probe is that the substrate to be tested can initiate the reaction between two molecular fragments, and then the push-pull electron conjugated chain of the dye is assembled in situ in the detection system and is generated A highly sensitive signal.A variety of such probes have been reported before our group proposed the concept of “covalent assembly.” In this paper, different types of probes with “covalent assembly” Features of the typical probes and detection reagents were summarized.