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目的:观察侧柏炭黄酮类成分群对干酵母致血热复合出血模型大鼠的止血作用。方法:对照组和模型组灌胃CMCNa,以云南白药为阳性药,阳性组、剔除前组、剔除后组、成分群低剂量组和成分群高剂量组分别灌胃相应的治疗药物,连续给药8 d,于第7天复制出血热复合出血模型,以活化部分凝血活酶时间(APTT)、血浆凝血酶时间(TT)、凝血酶原时间(PT)、纤维蛋白原的量(FIB)、血小板计数(PLT)、血小板分布宽度(PDW)、平均血小板体积(MPV)、ADP诱导的血小板聚集率、血栓素(TXB2)、6-酮前列腺素(6-keto-PGF1α)、红细胞计数(RBC)、红细胞压积(HCT)、血红蛋白(HGB)的量、全血黏度、环氧合酶2(COX-2)等评价指标为导向,考察侧柏炭乙酸乙酯部位成分群的止血作用。结果:与对照组比较,模型组大鼠APTT,TT显著延长(P<0.01,P<0.05),PT显著缩短(P<0.05),FIB的量明显增加(P<0.01);PLT,PDW,MPV显著增加(P<0.01,P<0.05),ADP诱导的血小板聚集率显著降低(P<0.05),6-keto-PGF1α显著降低(P<0.01),TXB2显著升高(P<0.01);各切变率下全血黏度明显增加(P<0.01),RBC,PLT和HGB明显升高(P<0.01),C0X-2的表达量显著升高(P<0.01)。黄酮类成分群可明显缩短APTT,TT(P<0.01,P<0.05),明显降低FIB的量(P<0.05);明显降低PDW和PLT(P<0.01,P<0.05),显著提高ADP诱导的血小板聚集率(P<0.05),6-keto-PGF1α显著升高(P<0.01,P<0.05),TXB2显著降低(P<0.01);明显降低模型大鼠RBC、HCT、HGB及全血低切黏度(P<0.01,P<0.05),C0X-2的表达量显著降低(P<0.01),改善肺组织病理性出血现象。剔除后组对模型大鼠各环节的作用较弱。结论:侧柏炭黄酮类成分群主要通过改善内源性凝血功能及促进血小板聚集功能、降低全血低切黏度等发挥其凉血止血作用,是侧柏炭止血有效部位。
Objective: To observe the hemostatic effect of Aristolochia orientalis flavonoids on hemorrhagic fever with hemorrhagic fever caused by dry yeast. Methods: The control group and model group were administered with CMCNa, Yunnanbaiyao as positive drug, positive group, the former group, the latter group, the low dose group and the high dose group were given the corresponding therapeutic drugs, continuous On the 7th day, the hemorrhagic fever combined with hemorrhage model was replicated on the 7th day to activate the APTT, TT, PT, FIB, , PLT, PDW, MPV, ADP-induced platelet aggregation, TXB2, 6-keto-PGF1α, (RBC), hematocrit (HCT), hemoglobin (HGB), whole blood viscosity and cyclooxygenase 2 (COX-2) . Results: Compared with the control group, APTT, TT in model group were significantly prolonged (P <0.01, P <0.05), PT was significantly shortened (P <0.05), FIB was significantly increased (P <0.01) MPV significantly increased (P <0.01, P <0.05), ADP-induced platelet aggregation decreased significantly (P <0.05), 6-keto-PGF1α significantly decreased (P <0.01) The whole blood viscosity increased significantly at each shear rate (P <0.01), RBC, PLT and HGB were significantly increased (P <0.01), and the expression of COX-2 was significantly increased (P <0.01). The content of flavonoids could significantly reduce APTT, TT (P <0.01, P <0.05) and significantly reduce the amount of FIB (P <0.05); significantly reduce PDW and PLT (P <0.01, P < (P <0.01, P <0.05), TXB2 decreased significantly (P <0.01), and the levels of RBC, HCT, HGB and whole blood in model rats decreased significantly (P <0.05) Low shear viscosity (P <0.01, P <0.05), C0X-2 expression was significantly reduced (P <0.01), improve the phenomenon of pulmonary hemorrhage. After removing the group on the role of the model rats weaker. Conclusion: The main components of oriental arborvitae carbon flavonoids are the effective parts of Arborvitae carbon hemostasis, which are mainly through improving the function of endogenous coagulation, promoting platelet aggregation and decreasing the viscosity of whole blood.