【摘 要】
：
为克隆和原核表达牛边缘无浆体(A.marginale)膜表面蛋白2(MSP2),并分析其免疫原性,本研究通过PCR方法扩增A.marginale的MSP2基因,将其连接到pGEX 6p-1载体中,转化感受态细胞B
【机 构】
：
黑龙江八一农垦大学动物科技学院,黑龙江大庆163319吉林农业大学动物科技学院,吉林长春130118;黑龙江省鹤山农场鹤澳奶牛繁育中心,黑龙江齐齐哈尔,161443;黑龙江八一农垦大学动物科技学院,黑
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为克隆和原核表达牛边缘无浆体(A.marginale)膜表面蛋白2(MSP2),并分析其免疫原性,本研究通过PCR方法扩增A.marginale的MSP2基因,将其连接到pGEX 6p-1载体中,转化感受态细胞BL21 (DE3),经IPTG诱导表达,进行SDS-PAGE分析,将纯化的MSP2重组蛋白免疫小鼠.结果表明表达并纯化了A.marginale MSP2,其免疫原性良好,为制备单克隆抗体及亚单位疫苗的研制奠定了基础.
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