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目的观察皇家外科学院大鼠(royal college of surgeons,RCS)视网膜变性过程中的暗适应视网膜电图(electroretinogram,ERG)振荡电位(oscillatory potentials,OPs)频域特性。方法选择出生后20、30、40 d及60 d的RCS大鼠各3只,采用RETI-scan记录系统进行闪光视网膜电图(flash electroretinogram,F-ERG)OPs记录,记录电极为环形角膜电极,参考电极为不锈钢针状电极,刺激强度为0dB,通过Matlab提取OPs成分,分析其频谱特性。结果相对于同龄的正常大鼠,RCS大鼠OPs频域幅值明显重度降低(P<0.01),且高频成分缺失。RCS大鼠发育过程中,随着病程进展至40 d时对应的频率向高频部分明显延迟(P<0.05),进展至60 d时其频域幅值明显降低(P<0.05)。结论 RCS大鼠ERG的OPs频域特性与视网膜功能的变性程度存在相关性,利用这些特性可以更明显观察到视网膜变性对大鼠视网膜功能的影响。
Objective To observe the frequency-domain characteristics of dark-adapted electrostimulatory (OPG) oscillatory potentials (ERs) in the process of retinal degeneration in rats of royal college of surgeons (RCS). Methods Three RCS rats were selected at 20, 30, 40, and 60 days after birth. The OPs were recorded by flash electroretinogram (F-ERG) using the RETI-scan system. The recording electrodes were corneal electrodes, The reference electrode is a stainless steel needle electrode, the stimulus intensity is 0dB, and the components of OPs are extracted by Matlab to analyze the spectral characteristics. Results Compared with normal rats of the same age, the amplitudes of OPs in RCS rats decreased significantly (P <0.01) and the high frequency components were absent. During the development of RCS rats, the corresponding frequency was delayed to the high frequency part (P <0.05) as the disease progressed to 40 days, and the amplitude amplitude of the RCS rats decreased significantly (P <0.05) at 60 days. Conclusion There is a correlation between the OPs frequency domain characteristics and the degree of degeneration of retinal function in RCS rat ERG. The retinal degeneration can be observed more clearly by using these characteristics.