论文部分内容阅读
目的 :探讨 β -榄香烯的抗癌疗效及其作用机制。方法 :采用MTT方法、Hoechst3334 2和PI荧光染色法、电镜及流式细胞术分析法 ,发现 β-榄香烯乳剂对K562 细胞生长的影响。结果 :β -榄香烯明显抑制K562 细胞的生长 ,对K562 细胞的半数生长抑制剂量 (IC50 )为 17.14μg/ml。其抑制细胞生长能力主要是通过诱导细胞凋亡 ,并且呈浓度和时间依赖性。在影响细胞周期方面 ,主要使G1期细胞数目增多 ,S期细胞数目下降。结论 :β -榄香烯诱导细胞凋亡是其抗肿瘤作用机制的重要方面之一。
Objective: To explore the anti-cancer efficacy and mechanism of β-elemene. METHODS: The effects of β-elemene emulsion on the growth of K562 cells were detected by MTT assay, Hoechst 3334 2 and PI fluorescence staining, electron microscopy and flow cytometry analysis. RESULTS: β-elemene significantly inhibited the growth of K562 cells, and the IC50 of K562 cells was 17.14 μg/ml. Its ability to inhibit cell growth is primarily induced by apoptosis, and is concentration- and time-dependent. In terms of affecting the cell cycle, the number of cells in the G1 phase is mainly increased, and the number of cells in the S phase is decreased. Conclusion :β-elemene induced apoptosis is one of the important aspects of its anti-tumor mechanism.