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目的:评价新研制的系列5-氨基酮戊酸衍生物光动力抗肿瘤作用。方法:(1)制备系列新型5-氨基酮戊酸衍生物;(2)对新化合物经细胞孵育转化成的原卟啉Ⅸ进行紫外吸收光谱与荧光发射光谱测定:将新化合物经7 721细胞孵育5 h后,用紫外分光光度计和荧光光谱仪测定其特征峰。(3)MTT法:将不同浓度的新化合物与7 721细胞共孵育5 h后,用不同剂量的635 nm激光照射,MTT法检测细胞存活率。(4)PDT治疗小鼠肿瘤:以100 mg/kg的给药剂量对已成功接种
OBJECTIVE: To evaluate the photodynamic anti-tumor effects of a series of 5-aminolevulinic acid derivatives newly developed. Methods: (1) Preparation of a series of new derivatives of 5-aminolevulinic acid; (2) UV spectrophotometry and fluorescence emission spectrometry of protoporphyrin Ⅸ, which was transformed into new compounds by cell incubation, After incubation for 5 h, the characteristic peaks were measured by UV spectrophotometer and fluorescence spectrometer. (3) MTT method: Different concentrations of new compounds incubated with 7 721 cells for 5 h, with different doses of 635 nm laser irradiation, MTT assay cell viability. (4) PDT treatment of mouse tumors: 100 mg / kg dose of the pair has been successfully vaccinated