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目的研究西沙海绵Agelas aff.nemoechinata的化学成分。方法利用Sephadex LH-20凝胶色谱、硅胶色谱、ODS色谱、HPLC等手段对化学成分进行了分离纯化;通过理化性质,波谱分析方法结合文献对照,鉴定化合物的结构。结果从南海海绵甲醇溶剂提取物中,分离鉴定了8个单体化合物:尿嘧啶(1)、胸腺嘧啶(2)、1 H-2-吡啶酮(3)、胸腺嘧啶-2’-脱氧核苷(4)、2-甲酰胺吡咯(5)、3-醛基吲哚(6)、6-羟基-4,4,7a-三甲基-5,6,7,7a-四氢苯并呋喃-2(2 H)酮(7)、5-氨基戊酸(8)。结论化合物1~8均为首次从该种海绵中分离得到。化合物7在50μg·mL-1浓度水平,对K562白血病细胞株、P388白血病细胞株和HeLa肿瘤细胞株的抑制率分别是38.29%、23.23%和95.57%。
Objective To study the chemical constituents of Agelas aff.nemoechinata in Xisha sponge. Methods The chemical constituents were separated and purified by Sephadex LH-20 gel chromatography, silica gel chromatography, ODS chromatography and HPLC. The structures of the compounds were identified by physico-chemical analysis and spectroscopic analysis. Results Eight monomer compounds, uracil (1), thymine (2), 1H-2-pyridone (3), thymine-2’-deoxyribonucleic acid (4), 2-carboxamidopyrrole (5), 3-aldehyde indole (6), 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenz Furan-2 (2H) -one (7), 5-aminopentanoic acid (8). Conclusion Compounds 1 to 8 were isolated from this sponge for the first time. The inhibitory rates of compound 7 at the concentration of 50μg · mL-1 for K562 leukemia cell line, P388 leukemia cell line and HeLa tumor cell line were 38.29%, 23.23% and 95.57%, respectively.