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功物肾上腺皮质主要分泌皮质酮,至于使用放射免疫方法测定动物血浆皮质醇的研究,尚缺乏应有的实验资料。毫无疑问,研究这一问题,对于了解动物肾上腺皮质的功能,对阐明调节原理,都具有一定的理论意义。本文采用北京化工厂生产供应的~(125)Ⅰ—皮质醇试剂药盒,测定了家兔、大白鼠和狗的血浆皮质醇正常值,以及对家兔进行了促肾上腺皮质激素(ACTH)兴奋试验。现将结果报告如下。方法一、实验动物1、家兔50只(雄性24只,雌性26只),体重2—3公斤。于上午8时耳静脉取血。2、大白鼠50只(雄、雌各25只),体重290—470克。于上午8时心脏取血(从抓动物至取血在5分钟内完成)。
The adrenal cortex mainly secretes corticosterone. As for the study of animal plasma cortisol using radioimmunoassay, there is still lack of experimental data. There is no doubt that studying this issue has certain theoretical significance for understanding the function of adrenal cortex in animals and elucidating the principle of regulation. This article uses the ~(125)I-cortisol reagent kit manufactured and supplied by Beijing Chemical Factory to determine the normal plasma cortisol levels in rabbits, rats, and dogs, and to stimulate ACTH in rabbits. test. The results reported below. Method 1. Experimental animals 1. 50 rabbits (24 males and 26 females) weighing 2-3 kg. Blood was collected from the ear vein at 8 a.m. 2. 50 rats (25 males and females) weighing 290-470 grams. At 8 o’clock in the morning, the heart took blood (from scratching the animal to taking blood within 5 minutes).