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应用经酶和机械消化分离的大鼠背根神经节（ＤＲＧ）神经元进行实验。首先运用全细胞膜片箝技术找到ＮＭＤＡ可引起一内向电流的神经元，此内向电流可被甘氨酸明显增强，并被ＮＭＤＡ受体的特异性拮抗剂ＡＰＶ完全阻断。随后，把经电生理技术证实膜上存在ＮＭＤＡ受体的这一ＤＲＧ神经元用微吸管转移到载玻片上，进行谷氨酸神经递质免疫组织化学检测。１０／１８的ＤＲＧ细胞呈免疫反应阳性，结果为ＤＲＧ神经元膜上存在ＮＭＤＡ自身受体提供了新证据。
Rat dorsal root ganglion (DRG) neurons isolated by enzymatic and mechanical digestion were used for experiments. First, using whole-cell patch clamp technique to find NMDA neurons can cause an inward current, the inward current can be significantly enhanced glycine, and NMDA receptor antagonist APV completely blocked. Subsequently, DRG neurons confirmed by electrophysiological techniques to have NMDA receptors on the membrane were transferred to slides with a micropipette for immunohistochemical detection of glutamate neurotransmitter. DRG cells in 10/18 were immunoreactive and the results provided new evidence for the presence of NMDA autoreceptors on DRG neuronal membranes.