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目的了解南宁市朝阳溪污水中诺如病毒污染状况。方法 2011年1-12月采集72份南宁市朝阳溪污水,用混合纤维素膜吸附法吸附病毒和聚乙二醇(PEG)沉淀法浓缩病毒后,经实时荧光逆转录-聚合酶链(Real-time RTPCR)方法进行NV核糖核酸(RNA)检测。结果全年采集72份污水中,诺如病毒核酸阳性率为100.00%,其中基因Ⅰ组阳性数为51份,阳性率为70.83%,基因Ⅱ组阳性数为72份,阳性率为100.00%。基因Ⅰ组冬春季检出率高于其他季节,基因Ⅱ组检出率无季节差异。结论南宁市朝阳溪污水中存在基因Ⅰ组和Ⅱ组诺如病毒污染,检出率高的季节与相关NV引起的腹泻高发季节报道相一致,污水中诺如病毒的监测是人群监测手段的重要补充。
Objective To understand the status of norovirus infection in Chaoyangxi wastewater in Nanning. Methods Seventy-two sewage samples from Chaoyangxi of Nanning City from January to December in 2011 were collected by mixed cellulose membrane adsorption method and polyethylene glycol (PEG) precipitation method. After real-time fluorescence reverse transcription-polymerase chain reaction -time RTPCR method for the detection of NV ribonucleic acid (RNA). Results The positive rate of norovirus was 100.00% in 72 semen collected in the whole year, of which 51 were positive in group Ⅰ, the positive rate was 70.83%, and 72 in positive group Ⅱ, the positive rate was 100.00%. The detection rate of gene Ⅰ in winter and spring was higher than that in other seasons, and there was no seasonal difference in the detection rate of gene Ⅱ. Conclusions The genotype Ⅰ and group Ⅱ Norovirus infection in Chaoyangxi wastewater in Nanning City is high. The season with high detection rate is consistent with the seasonal reports of high incidence of diarrhea caused by NV. The monitoring of norovirus in wastewater is important for monitoring the population supplement.