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目的探寻职业性锰接触工人早期较敏感的接触生物检测指标。方法选择锰铁合金生产企业163名男职工作为暴露组(其中高浓度接触组85人,低浓度接触组78人);选择体力劳动强度相近的生铁冶炼厂男职工80人作为对照组。采用石墨炉原子吸收分光光度法测定研究人群生物样本中锰的含量,用火焰原子吸收分光光度法测定空气中锰。结果高剂量接触组唾液锰为32.32μg/L、血浆锰为29.96μg/L、血细胞中锰为15.12ng/g,明显高于低剂量接触组和对照组(P<0.01),而不同接触剂量组之间尿锰和发锰含量的差异没有统计学意义;在工龄<5a的接触人群中唾液和血细胞中锰的含量与环境锰浓度有很好的一致性,在工龄≥5a的接触人群中,虽然高剂量接触组的唾液、血浆和血细胞中的锰含量高于低剂量接触组,但增加的趋势低于工龄<5a的接触人群。结论由于唾液采集无创伤,唾液锰可作为锰接触工人早期生物标志物。
Objective To explore the sensitive early detection of contact biomarkers in occupational manganese exposed workers. Methods A total of 163 male employees of ferro-manganese alloy production enterprises were selected as the exposure group (85 in the high-concentration exposure group and 78 in the low-concentration exposure group), and 80 male workers in pig iron smelters with similar manual labor intensity were selected as the control group. Graphite furnace atomic absorption spectrophotometry was used to determine the content of manganese in the biological samples of the population, and the content of manganese in the air was determined by flame atomic absorption spectrophotometry. Results In the high-dose exposure group, saliva manganese was 32.32 μg / L, plasma manganese was 29.96 μg / L, and manganese in blood cells was 15.12 ng / g, which was significantly higher than that of the low-dose exposure group and the control group (P <0.01) There was no significant difference in urinary manganese and manganese content among the groups. The content of manganese in saliva and blood cells in working population <5a was in good agreement with the environmental manganese concentration. In the exposed population of ≥5 years Although the content of manganese in saliva, plasma and blood cells of high-dose exposure group was higher than that of low-dose exposure group, the trend of increase was lower than that of contact age <5a. Conclusion Due to the noninvasive saliva collection, saliva manganese can be used as an early biomarker for exposure of workers to manganese.