论文部分内容阅读
随着市场的需要 ,商品种子量越来越大 ,而对杂交种质量问题不容忽视 ,我们在实际工作中对中椒四号 ,中椒五号 ,京欣一号 ,金花宝四个杂交种纯度鉴定尝试了大量的同工酶电泳方法 ,包括 POX,EST,ADH,MDH,ISH,PGM,PGI等同工酶电泳图谱中均未找到父母本与一代杂种的鉴别标记。而在种子蛋白质 SDS- PAGE电泳图谱中找到了一代杂种与父母本的鉴别带 ,并利用这个鉴别带进行了杂交种纯度鉴定。这种鉴定方法的准确性在田间对照纯度鉴定中得到确认 ,此方法可用于杂交种商品种子的纯度检测
With the needs of the market, the amount of commodity seeds is growing, but the quality of hybrids can not be ignored, we in the actual work Zhongjiao IV, Zhongjiao No. 5, Jinxin one, Jinhua Bao four hybrids Purity identification A large number of isozyme electrophoresis methods were attempted, including no identification of parents and F1 hybrids in isoelectric patterns of POX, EST, ADH, MDH, ISH, PGM and PGI. In the SDS-PAGE electrophoresis pattern of seed protein, the identification bands of the first generation hybrids and parents were found, and the purity of the hybrids was identified by using this identification band. The accuracy of this method is confirmed in the field of control purity identification, this method can be used for hybrid seed purity testing