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利用蜂毒治疗类风湿性关节炎(RA)已有悠久的历史,并取得了良好的治疗效果.为研究其抗炎机制及确定有效的抗炎成分,利用凝胶过滤层析、肝素亲和柱层析、高效液相色谱等方法分离蜂毒多肽,并观察蜂毒多肽对小鼠脾淋巴细胞细胞周期、细胞因子合成及IκBα(蛋白磷酸化的影响.高效液相色谱检测分离得到的蜂毒多肽BVⅠ-2H为单一对称峰,电喷雾质谱检测结果表明BVⅠ-2H是蜂毒多肽混合物,其主要成分的分子量为644.8 u.BVⅠ-2H可抑制ConA诱导的小鼠脾淋巴细胞增殖和IL-1合成,可使ConA诱导的脾淋巴细胞呈现明显的G2/M期阻滞.此外,BVⅠ-2H可抑制PMA诱导THP-1细胞合成TNFα,抑制TNFα mRNA的表达及IκBα蛋白磷酸化.实验结果提示,蜂毒多肽BVⅠ-2H是蜂毒发挥抗炎作用的重要组成成分.
The use of bee venom for the treatment of rheumatoid arthritis (RA) has a long history and has achieved a good therapeutic effect. In order to study its anti-inflammatory mechanism and determine effective anti-inflammatory components, the use of gel filtration chromatography, heparin affinity column chromatography, high performance liquid chromatography and other methods to isolate bee venom peptides, and observe the bee venom peptides on mouse spleen lymphocytes Cell Cycle, Cytokine Synthesis, and IκBα (Influence of Protein Phosphorylation. The isolated bee venom polypeptide BVI-2H was detected as a single symmetrical peak. Electrospray ionization mass spectrometry indicated that BVI-2H was a mixture of bee venom peptides. The molecular weight of the main component is 644.8 u.BVI-2H can inhibit ConA-induced proliferation of spleen lymphocytes and IL-1 synthesis in mice, and ConA-induced splenic lymphocytes show a clear G2/M phase arrest. BVI-2H can inhibit PMA-induced THP-1 cells to synthesize TNFα, inhibit the expression of TNFα mRNA and phosphorylation of IκBα protein. The experimental results suggest that bee venom polypeptide BVI-2H is an important component of bee venom to exert anti-inflammatory effects.