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目的:调查云南省昆明市动物园和宠物市场的鹦形目鸟类中鹦鹉热衣原体的流行情况。创新点:首次在云南地区开展鹦形目鸟类中鹦鹉热衣原体的流行病学调查,发现该地区鹦形目鸟类中具有较高的鹦鹉热衣原体感染率。方法:本研究共采集8种鹦形目鸟类的新鲜粪便样本136份,其中动物园样本60份,宠物市场样本76份。首先,利用高灵敏和高特异性的TaqMan MGB探针荧光定量聚合酶链反应(qPCR)方法检测粪便样品中鹦鹉热衣原体的感染率,然后针对阳性样品,利用PCR技术进行鹦鹉热衣原体OmpA基因扩增、纯化、测序以及基因型分析。结论:本实验结果显示:鹦鹉热衣原体的感染率为19.9%(27/136),其中宠物市场鸟类中鹦鹉热衣原体的感染率(26.3%;20/76)明显高于动物园(11.7%;7/60)(P=0.034);金刚鹦鹉中感染率最高,达41.2%(7/17)。同缘关系进化树分析表明,这些鹦鹉热衣原体都属于易于由鸟向人跨物种传播的A型。综上所述,昆明地区鹦形目鸟类中具有较高的鹦鹉热衣原体流行,给饲养人员和观鸟者的健康带来了潜在的威胁。
OBJECTIVE: To investigate the prevalence of Chlamydia psittaci in the zooplankton at the Kunming Zoo and Pet Market in Yunnan Province. Innovative point: Epidemiological investigation of Parrotchinum chlamydia in the genus of avian was first conducted in Yunnan region. It was found that there was a high infection rate of chlamydia psittaci in the region. Methods: A total of 136 fresh stool samples from 8 species of ornithopterygium were collected in the study, including 60 zoo samples and 76 pet samples. First, the infection rate of Chlamydia psittaci in stool samples was detected by TaqMan MGB probe with high sensitivity and specificity. Then, the positive rate of OmpA gene amplification of Chlamydia psittaci Purification, sequencing, and genotyping. Conclusion: The results showed that the infection rate of Chlamydia psittaci was 19.9% (27/136). The prevalence of Chlamydia psittaci (26.3%; 20/76) in the pet market was significantly higher than that in the zoo (11.7%; 7/60) (P = 0.034). The highest infection rate in macaques was 41.2% (7/17). Phylogenetic tree analysis shows that these chlamydia psittaci all belong to type A which are easily transmitted from bird to person across species. To sum up, the endemic birds of the genus Porphyrio in Kunming have a high prevalence, posing a potential threat to the health of feeders and birdwatchers.