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目的:探讨丹红注射液中中药单体丹酚酸B(Sal B)及羟基红花黄色素A(HSYA)小复方对神经元糖氧剥夺(OGD)损伤的保护作用,及其抗自由基损伤的交互作用,优化配比组合。方法:体外培养SD大鼠海马神经元,考虑A(是否OGD造模)、B(Sal B,25,50,75μmol·L-1)及C(HSYA,20,40,60μmol·L-1)3个因素,采用完全随机分组的2×3×3析因设计,倒置相差显微镜观察细胞形态,采用黄嘌呤氧化酶法测定细胞内超氧化物歧化酶(SOD)活性。结果:A,B,C三因素均对SOD活性具有极显著影响(P<0.01)且B,C及A,B间存在交互作用(P<0.01)。以Sal B 75μmol·L-1,HSYA 60μmol·L-1时,SOD活力最高。结论:Sal B与HSYA合用在抗自由基损伤方面对缺血缺氧再灌注损伤的海马神经元具有协同保护作用。
Objective: To explore the protective effect of Salviae Miltiorrhizae (Bombyx Mori) and compound HSYA on the damage of OGD in Danhong injection and its anti-free radical Injury interaction, optimize the ratio of combinations. Methods: The hippocampal neurons of SD rats were cultured in vitro. A (whether OGD modeling), B (Sal B, 25, 50 and 75μmol·L-1) and C (HSYA, 20, 40 and 60μmol·L-1) Three factors were randomly divided into 2 × 3 × 3 factorial designs. The morphological changes of cells were observed by inverted phase contrast microscope. The activity of superoxide dismutase (SOD) in the cells was measured by xanthine oxidase method. Results: Three factors, A, B and C, had a very significant effect on SOD activity (P <0.01) and there was interaction between B, C and A, B (P <0.01). With Sal B 75μmol·L-1, HSYA 60μmol·L-1, SOD activity was the highest. Conclusion: Combination of Sal B with HSYA has a synergistic protective effect on hippocampal neurons against ischemia-reperfusion injury in anti-free radical injury.