目的:建立补肾温肺合剂的质量控制方法。方法:用薄层色谱法对补肾温肺合剂中黄芪进行定性鉴别;采用高效液相色谱法测定补肾温肺合剂中淫羊藿苷的含量。结果:薄层色谱中斑点清晰,易于识别;淫羊藿苷线性范围为0.20～4.08μg(r=0.999 8),平均回收率为98.88%,RSD=1.27%。结论:本方法简便,灵敏度高,可有效地控制补肾温肺合剂的质量。 Objective: To establish a method of quality control of Bushen Wenfei Mixture. Methods: TLC was used to qualitatively identify Radix Astragali in Bushen Wenfei Mixture. The content of icariin in Bushen Wenfei Mixture was determined by high performance liquid chromatography. Results: The spots in TLC were clear and easy to identify. The linear range of icariin was 0.20 ~ 4.08μg (r = 0.999 8). The average recovery was 98.88% and RSD was 1.27%. Conclusion: The method is simple, high sensitivity, which can effectively control the quality of Bushen Wenfei Mixture.