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目的:研究131I标记的抗肾癌相关抗原G250单克隆抗体(131I-G250McAb)在荷人肾透明细胞癌裸鼠体内的分布,探讨应用131I-G250单克隆抗体治疗实验性人肾癌的可行性和有效性。方法:将标记好的131I-G250单克隆抗体1mL/只(3.7MBq)静脉注射荷人肾癌裸鼠体内,后分批处死裸鼠,测定每克组织摄取占放射性计数百分比(%ID/g),肿瘤与其他脏器的单位质量放射性比值(T/NT)获得131I-G250单克隆抗体在体内的分布特点;取16只裸鼠根据性别、体质量和肿瘤体积大小随机分配到A、B、C和D 4个不同的处理组中,1个月后测量各组不同时点肿瘤体积大小。结果:131I-G250抗体分布在肿瘤组织中始终处于较高浓度,T/NT最大值为5(肿瘤/肌肉),48h后平均每克肿瘤组织%ID/g达最大值为8.61±0.29。30d后肿瘤体积A、B、C和D组分别为(2.04±0.18)、(0.77±0.04)、(1.85±0.11)和(1.81±0.22)cm3,B组肿瘤体积与其他3组比较差异有统计学意义,F=76.14,P<0.05。结论:用131I标记G250单克隆抗体在实验动物体内的肿瘤部位存在特异的浓聚并能有效抑制肿瘤的生长。
Aims: To investigate the distribution of 131I-labeled monoclonal antibody against renal cell carcinoma-associated antigen G250 (131I-G250McAb) in human renal clear cell carcinoma in nude mice and to explore the feasibility of using 131I-G250 monoclonal antibody to treat human renal cell carcinoma And effectiveness. Methods: The labeled 131I-G250 monoclonal antibody (1 mL / mouse) (3.7 MBq) was injected intravenously into nude mice bearing human renal cell carcinoma and the nude mice were sacrificed in batches. The percentage of radioactivity per gram of tissue taken up (% ID / ), Tumor and other organs of the unit mass radioactivity ratio (T / NT) 131I-G250 monoclonal antibody in vivo distribution characteristics; take 16 nude mice according to gender, body mass and tumor size were randomly assigned to A, B , C and D four different treatment groups, one month after the measurement of tumor volume at different time points in each group size. Results: The distribution of 131I-G250 antibody in the tumor tissue was always at a higher concentration with a maximum T / NT of 5 (tumor / muscle), and the maximum value of% ID / g per gram of tumor tissue after 48h was 8.61 ± 0.29.30d (2.04 ± 0.18), (0.77 ± 0.04), (1.85 ± 0.11) and (1.81 ± 0.22) cm3 in groups A, B, C and D, respectively. The difference of tumor volume in group B was statistically significant compared with the other three groups Significance, F = 76.14, P <0.05. CONCLUSION: The 131I-labeled G250 monoclonal antibody has specific concentration in the tumor site of experimental animals and can effectively inhibit tumor growth.