海藻/昆布粗多糖的经口亚急性毒性及影响肝药酶特性的研究

来源 :上海海洋大学学报 | 被引量 : 0次 | 上传用户:tastgaoyan1981
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
本研究从海藻/昆布药对临床应用的角度出发,以海藻/昆布药对的粗多糖组分为研究对象,探讨了药对粗多糖对大鼠的经口亚急性毒性以及对肝药酶活性的影响。通过试验设计,以雌雄各半SD大鼠连续经口给药2周不同剂量的药对粗多糖,记录给药期间大鼠生理状态和行为变化,末次给药后收集心、肝、脾、肺、肾等脏器做HE染色切片,光镜下观察组织病理学变化,并以探针底物体外温孵法测定给药后大鼠肝药酶活性变化。结果发现,给药后低剂量处理组[10.8 g/(kg·d)]和高剂量处理组[86.4 g/(kg·d)]大鼠的生理体征及脏器系数未见显著异常。在显微结构下,低剂量组各脏器形态基本正常,而高剂量组肝组织出现水肿、脂肪变及肝窦扩张,肺脏组织出现间质性炎症及气肿。低剂量组大鼠肝药酶活性未见异常,高剂量组肝药酶CYP3A4活性被显著诱导。由此推测,海藻/昆布粗多糖具有潜在细胞毒性,大剂量下可致肝、肺组织毒性损伤,并可能通过诱导肝药酶CYP3A4发生药物与药物的相互作用,最终影响药物的药效或产生不良反应等。 In this study, from the point of view of clinical application of seaweed / kelp medicine, the crude polysaccharides components of seaweed / kelp were used as the research object to investigate the oral subacute toxicity of crude polysaccharides to rats and the activity of hepatic drug Impact. Through experimental design, the male and female SD rats were orally administered with different dosages of crude polysaccharides continuously for 2 weeks. The physiological status and behavior of the rats during the administration were recorded. Heart, liver, spleen and lungs were collected after the last administration , Kidney and other organs were stained with HE, histopathological changes were observed under light microscope, and the change of enzyme activity in rat liver after administration was detected by probe substrate incubation in vitro. The results showed that there was no significant difference in the physical signs and organ coefficients between the low-dose group [10.8 g / (kg · d)] and the high-dose group [86.4 g / (kg · d)] after administration. Under the microstructure, the morphology of the organs in the low-dose group was basically normal, while the edema, steatosis and hepatic sinusoid expansion in the liver tissue of the high-dose group appeared, interstitial inflammation and emphysema in the lung tissue. The liver enzyme activity of rats in low dose group showed no abnormality, and the activity of hepatic drug CYP3A4 in high dose group was significantly induced. It is speculated that seaweed / laminaria crude polysaccharides have potential cytotoxicity, high doses can cause liver and lung toxicity, and may induce liver drug CYP3A4 drug and drug interactions occur, and ultimately affect the drug’s efficacy or produce Adverse reactions.
其他文献
9月8日中国灭火队在科威特扑灭了第一口油井大火,受到了科方的赞扬。那么,中科是如何签订灭火合同的呢?中国石油工程建设总公司副总经理赵焕国披露了有关情况。海湾战争结束
利用脉冲激光沉积法成功制备了BaTiO3/p-Si异质结,该异质结在80—300K显示出了良好的整流特性和光诱导特性.开启电压随着温度的升高而逐渐降低.利用不同频率的光子辐照样品,
既然是宝马,就先了解下它的驾驶感受吧,虽然它是一款十足的高级商务用车。对于7系的驾驶者来讲,这次最大最直观的变化是换挡把从方向盘后面移到了传统的位置,并且采用了与×5
叙利亚1989、1990年总产气量分别为15.5亿m~3和20亿m~3。预计1991、1993年总产气量将分别为23亿m~3和27亿m~3。到1991年,天然气加工厂将达到3个。苏韦迪耶(Suwaidiyah)加工厂日
目的以亚叶酸钙(LV)为对照药,评价左亚叶酸钙(L-LV)联合5-氟尿嘧啶(5-FU)、奥沙利铂(L-OHP)治疗晚期或复发胃癌、结直肠癌的临床有效性和安全性。方法采用随机、多中心、平行
在苏联向炼油厂提供原油时的主要技术要求是对原油必要的处理程度。对此程度的要求在苏联国家标准9965—76《石油。石油加工企业的处理程度,技术条件》中有所规定。在一些主
探讨了淬火工艺对双金属带锯条齿部和背部材料的微观组织与力学性能、实际锯切寿命及断口形貌特征的影响.结果表明:淬火工艺对双金属带锯条背部与齿部材料的组织与力学性能有影
在已研制的含稀土Al2O3/TiCN陶瓷刀具材料的基础上,采用SEM,TEM和能谱分析等方法探讨了Y的强韧化效应。稀土增强Al2O3/TiCN陶瓷刀具材料力学性能改善的原因在于,Y的添加能在一定程
由大庆石油化工安装工程公司开发研制的新型防腐管道——钢塑复合管,是利用普通碳素钢管作基体,内衬化学稳定性优良的热塑性管,经机械复合加工成型的双层复合管道。它既 Th
目的研究丹参饮片高效液相(HPLC)指纹图谱,建立不同级别丹参饮片HPLC指纹图谱及其分级方法。方法运用戴安U3000-DAD-HPLC色谱仪,采用依利特C-18BP(4.6mm×250mm,5μm)柱,以0.