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设计、合成、表征以肌醇为六方向起始核的新型聚酰胺-胺树形高分子.第三代至第六代树形高分子(G3～G6)能够和DNA通过电荷相互作用形成聚电解质复合物,聚酰胺-胺树形高分子G6和荧光素酶基因(pRE Luc)形成的复合物的粒径为80～300 nm.G3～G6能介导半乳糖苷酶基因(pSV β-Gal)和荧光素酶基因有效转染HeLa和HEK293细胞.G5和G6的转染效率比聚赖氨酸高得多,接近枝化聚乙烯亚胺(branchedpolyethylenimine,PEI,Mw 25 K)的转染效率.G5和G6的细胞毒性低于聚-L-赖氨酸.
Design, synthesis and characterization of new polyamide-amine dendrimers with inositol in six directions.The third to sixth generations of dendrimers (G3 ~ G6) can interact with DNA to form poly Electrolyte complex, polyamide-amine dendrimer G6 and luciferase gene (pRE Luc) formed a particle size of 80 ~ 300 nm.G3 ~ G6 can mediate galactosidase gene (pSV β- Gal and luciferase genes were efficiently transfected into HeLa and HEK293 cells.The transfection efficiency of G5 and G6 was much higher than that of polylysine and close to the transfection of branched polyethylenimine (PEI, Mw 25 K) Efficiency .G5 and G6 cytotoxic than poly-L-lysine.