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目的了解深圳地区发热伴血小板减少综合征布尼亚病毒(SFTSV)的感染情况和人群特征,以及野鼠感染SFTSV的情况,为防治工作提供依据。方法采用ELISA法对91份疑似流行性出血热(EHF),且出血热Ig M抗体筛查阴性的住院患者样本以及在深圳市梧桐山捕获的64只野鼠进行SFTSV病毒血清学检测,同时应用实时荧光定量PCR法对SFTSV抗体筛查阳性者进行病毒的核酸检测。结果 91例流行性出血热Ig M抗体筛查阴性的患者中,发现5例SFTSV病毒急性感染者(4名男性和1名女性),SFTSV病毒Ig M抗体总阳性率为5.49%。感染者平均年龄为30.6岁,最小的19岁,最大的43岁。发现2例SFTSV病毒Ig G抗体阳性者,其中1例SFTSV病毒Ig M和Ig G抗体同时为阳性。此外,发现64例野鼠中有2例SFTSV病毒Ig G抗体阳性,均为褐家鼠。结论 2010年-2011年深圳地区的疑似流行性出血热住院人群中存在SFTSV急性感染和既往感染者,同时深圳地区褐家鼠存在SFTSV既往感染,应加强对疑似流行性出血热患者以及野鼠的SFTSV病毒的监测工作,进而为深圳地区发热伴血小板减少综合征(SFTS)的防控以及SFTS患者的诊断治疗提供依据。
Objective To investigate the prevalence and population characteristics of fever and with thrombocytopenic syndrome Bunyavirus (SFTSV) in Shenzhen and the status of SFTSV infection in voles to provide basis for prevention and treatment. Methods 91 samples of hospitalized patients with suspected epidemic hemorrhagic fever (EHF) and negative screening for hemorrhagic fever with IgM antibody and 64 wild animals captured in Wutongshan Mountain of Shenzhen were detected by ELISA. The results of real-time Fluorescent quantitative PCR method for SFTSV antibody screening positive for nucleic acid detection of the virus. Results Among the 91 patients with negative IgM antibody screening for epidemic hemorrhagic fever, 5 were found to be acute infection with SFTSV virus (4 males and 1 females). The positive rate of SFMV IgM antibody was 5.49%. The average age of infected persons is 30.6 years old, the youngest is 19 years old and the oldest is 43 years old. Two SFTSV-positive Ig G antibodies were found, of which one SFTSV-positive Ig M and Ig G antibodies were positive at the same time. In addition, SFTSV virus Ig G was found to be positive in 2 of 64 wild rodents, both Rattus norvegicus. Conclusion From 2010 to 2011, acute infection and previous infection of SFTSV were found in hospitalized patients with suspected epidemic hemorrhagic fever in Shenzhen. Meanwhile, there was a previous SFTSV infection in Rattus norvegicus in Shenzhen. SFTSV should be strengthened in patients with suspected epidemic hemorrhagic fever and in voles Virus monitoring work, and thus provide a basis for the prevention and treatment of fever with thrombocytopenic syndrome (SFTS) in Shenzhen and the diagnosis and treatment of SFTS patients.