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[目的]建立高效液相色谱-质谱/质谱法测定人参中精甲霜灵残留量的分析方法。[方法]采用外标法定量。检测条件如下:采用N-丙基乙二胺固相萃取柱(PSA),以0.1%甲酸水-乙腈为流动相梯度洗脱,提取时间为5 min,提取溶剂为丙酮,洗脱液为乙腈-甲苯混合溶液(体积比3∶1)。[结果]在选定的色谱质谱条件下,精甲霜灵的最低检出质量分数为0.002 mg/kg;方法的精密度为5.63%～15.62%;准确度为78.0%～89.1%。[结论]该方法符合农药残留检测技术的要求,适用于人参中精甲霜灵残留量的测定。
[Objective] To establish an HPLC-MS / MS method for the determination of metalaxyl residues in ginseng. [Method] Quantitative using external standard method. The detection conditions were as follows: N-propyl ethylenediamine solid phase extraction column (PSA), gradient elution with 0.1% formic acid in water and acetonitrile as eluent, the extraction time was 5 min, the extraction solvent was acetone and the eluent was acetonitrile - toluene mixed solution (volume ratio 3: 1). [Result] The minimum detectable mass fraction of metalaxyl was 0.002 mg / kg under the selected chromatographic conditions. The precision of the method was 5.63% ~ 15.62% and the accuracy was 78.0% ~ 89.1%. [Conclusion] The method met the requirements of pesticide residue detection technology and was suitable for the determination of metalaxyl residues in ginseng.