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为研究淋巴细胞在哮喘发病中的作用及地塞米松（Ｄｅｘ）、环孢菌素Ａ（ＣｓＡ）治疗哮喘的机理，用ＥＬＩＳＡ法检测哮喘患者和对照组的外周血单个核细胞（ＰＢＭＣ）在植物血凝素刺激下合成白细胞介素４（ＩＬ－４）、白细胞介素５（ＩＬ－５）水平，以及Ｄｅｘ和ＣｓＡ对哮喘患者的ＰＢＭＣ合成ＩＬ－４、ＩＬ－５的抑制作用。结果表明，哮喘组的ＩＬ－４、ＩＬ－５水平较对照组高（Ｐ＜０．０５）；１０－６ｍｏｌ／Ｌ的Ｄｅｘ和ＣｓＡ均能抑制哮喘组ＰＢＭＣ合成ＩＬ－４、ＩＬ－５，增大药物浓度至２×１０－６、３×１０－６ｍｏｌ／Ｌ时，ＣｓＡ对ＩＬ－５合成的抑制作用逐步增强；Ｄｅｘ对ＩＬ－５合成的抑制作用较ＣｓＡ强（Ｐ＜０．０５）。提示糖皮质激素和ＣｓＡ抑制ＩＬ－４、ＩＬ－５的合成是其治疗哮喘的机理之一。
To investigate the role of lymphocytes in the pathogenesis of asthma and the mechanism of dexamethasone (CsA) and cyclosporin A (CsA) treatment, peripheral blood mononuclear cells (PBMCs) were detected by ELISA in patients with asthma and controls IL-4 and IL-5 levels induced by phytohemagglutinin and the inhibitory effects of Dex and CsA on IL-4 and IL-5 production in PBMC of asthmatic patients. The results showed that the levels of IL-4 and IL-5 in the asthma group were higher than those in the control group (P <0.05). Both Dex and CsA at 10-6 mol / L inhibited IL-4 and IL- The inhibitory effect of CsA on IL-5 synthesis increased gradually when the drug concentration was increased to 2 × 10-6 and 3 × 10-6mol / L. The inhibitory effect of Dex on IL-5 synthesis was stronger than that of CsA (P <0.05 ). Suggest that glucocorticoid and CsA inhibit IL-4, IL-5 synthesis is one of the mechanisms of its treatment of asthma.