Differential Expression Analysis of Genie Male Sterility A/B Lines in Chinese Cabbage-Pak-Choi (Bras

来源 :Agricultural Sciences in China | 被引量 : 0次 | 上传用户:zeer
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
To determine differential expression of genie male sterility A/B lines in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee), we used the RNA fingerprinting technique, cDNA-AFLP analysis, in different developmental stages and different tissues. While no obvious differential expressions were observed in rosette leaves, florescence leaves, and scapes, some differential expressions were found in alabstrums of A/B lines and among leaves, scapes and alabstrums. We analyzed the al-abstrums collected in different developmental stages with 10 primer combinations. We got a unique band between middle size alabstrums and large alabstrums in B line in one of the ten pair primers, and in another one pair, one band reflecting a higher gene-expression level in A line than that in B line was obtained. No unique bands were found with the other primer combinations. The bands reflecting different gene-expression level were confirmed by Northern hybridization. The results indicat To determine differential expression of genie male sterility A / B lines in Chinese cabbage-pak-choi (Brassica campestris ssp. Chinensis Makino var. Communis Tsen et Lee), we used the RNA fingerprinting technique, cDNA-AFLP analysis, in different developmental stages and different tissues. While no obvious differential expressions were observed in rosette leaves, florescence leaves, and scapes, some differential expressions were found in abstrums of A / B lines and among leaves, scapes and alabstrums. We analyzed the al-abstrums collected in different developmental stages with 10 primer combinations. We got a unique band between middle size alabstrums and large absbs in B line in one of the ten pair primers, and in another one pair, one band reflecting a higher gene-expression level in A line than that in B line was obtained. No unique bands were found with the other primer combinations. The bands reflecting different gene-expression levels were confirmed by Northern hybridization. T he results indicat
其他文献
证明芝芪菌质对提高虾生产性能和机体免疫的作用。以日本沼虾为对象.进行试验,基础饲料中分别添加芝芪菌质。并与不添加芝芪菌质的对照组进行比较。试验期间,各试验组的水质基本
近年来,饲料添加剂在异育银鲫的养成中发挥着越来越重要的作用,如何科学使用饲料添加剂,使之既有利于鲫鱼的生长又对鲫鱼和人体没有副作用,是添加剂开发研究过程中需要不断探索的
在克氏原螯虾饲料中设置了0~8%共6个梯度的螺旋藻添加量,投喂成年及幼年虾,实验表明:(1)适量螺旋藻能促进螯虾生长和免疫能力提高,2%螺旋藻添加量对成虾增重率,不同虾龄螯虾含肉率提
会议
分别以动物蛋白:淀粉:纤维素为1:1:0、1:0:1、0:1:1、1:1:1的四种混合饲料喂养克氏原螯虾44d,并于10个时间点测定螯虾三种肠胃消化酶、SOD、糖原,结果显示:(1)饲料营养组分对螯虾肠胃相应
会议
徐州市濒临南四湖、骆马湖,境内河道纵横交错,水库星罗其布,据统计2007年水产养殖面积33.28万亩,产量163878t。由于水产养殖业发展迅猛,过高追求产量和效益,不注意自然生态平衡,病害
会议
需求分析随着我国经济的迅猛发展,当前专业AV技术的突飞猛进在最近这几年来的表现尤为突出,我司在AV系统的技术优势和丰富经验来设计本方案,在显示效果和扩声音效上体现用户
近几年,笔者在建设“两鱼两虾”特色渔业,带动全区水产养殖快速发展同时,遇到水产养殖病害制约产量、效益和产品质量提高的新情况。为此,笔者将通过分析养殖病害发生的原因,讨论水
本文论述了金鱼在规模化养殖过程中发生病害的发生季节性、种类的独特性、病害发生的快速性及防治难度性。指出了当前金鱼养殖病害防治的存在问题,提出了减少金鱼疾病的预防措
夏季是鱼类生长旺季,也是鱼类发病的高峰期。随着池塘养殖集约化程度和鱼产量的不断提高,鱼病的发生和造成的危害也越来越严重。目前本区夏季常见的鱼病有细菌性鱼病,病毒性鱼病
在当前渔业生产中,特别是淡水养殖中,其效益在没有绝对的保障情况下,如果一旦发生病害而造成鱼类死亡,那么,其效益将大大的受到影响,甚至亏损。笔者这几年在淡水鱼养殖技术服务中就