论文部分内容阅读
【目的】对塔克拉玛干沙漠腹地胡杨林土壤细菌多样性进行初步探索,为下一步从中筛选可用于生物饲料或生物肥料的微生物奠定基础。【方法】采用可培养方法,进行细菌的分离纯化。对各菌株进行革兰氏染色及淀粉酶、酯酶、纤维素酶和NaCl耐受浓度的测定,并提取各菌株基因组DNA,进行16SrRNA基因扩增、测序及系统进化树的绘制,分析其多样性。【结果】共分离得到27株菌,其中放线菌门(Actinobacteria)16株,变形菌门(Proteobacteria)4株,厚壁菌门(Firmicutes)6株,拟杆菌门(Bacteroidetes)1株。革兰氏染色结果表明,5株菌为革兰氏阴性,其余为革兰氏阳性;酶活测定结果表明,15株菌具有淀粉酶活性,9株菌具有酯酶活性,9株菌具有纤维素酶活性;NaCl耐受浓度测定结果显示,NaCl浓度为2%时所有菌株均能生长,5%时能生长的有22株,15%时能生长的有1株。【结论】塔克拉玛干沙漠腹地胡杨林土壤中存在较丰富的细菌类群,且具有一定的酶学活性和NaCl耐受性,具有进一步研究开发的价值。
【Objective】 The objective of this study was to explore the bacterial diversity of Populus euphratica forest in the Taklimakan Desert, and to lay the foundation for the further screening of microorganisms that can be used for biological feed or biological fertilizer. 【Method】 The culture method was used to separate and purify bacteria. Gram stain and determination of amylase, esterase, cellulase and NaCl tolerance of each strain were carried out. The genomic DNA of each strain was extracted and 16S rRNA gene was amplified, sequenced and phylogenetic tree was drawn. Sex. 【Result】 Twenty-seven strains were isolated, of which Actinobacteria 16, Proteobacteria 4, Firmicutes 6 and Bacteroidetes 1 were isolated. Gram staining results showed that the five strains were Gram-negative and the rest were Gram-positive. The results of enzyme activity assay showed that 15 strains had amylase activity, 9 strains had esterase activity, 9 strains had fiber The results of NaCl tolerance test showed that all strains could grow at 2% NaCl concentration, 22 strains grew at 5% and 1 strain grew at 15%. 【Conclusion】 The population of Populus euphratica forest in the hinterland of Taklimakan Desert is rich in bacterial groups, and has certain enzymatic activity and NaCl tolerance, which has the value of further research and development.