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目的 为了了解2 0 0 4年首发SARS病例周围环境鼠害及鼠类是否存在SARS CoV特异基因,研究探索鼠类作为SARS的媒介或来源的可能性。方法 应用环境鼠迹目测法与鼠笼诱法调查鼠密度方法和应用巢式RT PCR检测鼠样本中特异SARS CoV基因,扩增阳性产物测序,并应用DNASTAR软件分析比较。结果 首例病人居住工作的LJ花园LB楼及其周边楼宇环境鼠害较严重,重点检查首层楼房天花板、配电房等重点场所共5 0间,鼠迹阳性率为2 6 % ;检查住宅楼周围外环境累计2 0 0 0m ,发现鼠迹6 2处;鼠笼诱阳性率7.9% ;用RT PCR检测病例周围环境15只家鼠和2 4只臭鼠句鼠青的肺组织和肛拭子,其中6只黄胸鼠肺组织中有3只阳性,一只肛拭子阳性,其余为阴性;扩增阳性产物基因核苷酸序列与SARS目的基因序列的同源性为95 %及96 %。结论 2 0 0 4年首例SARS病例生活工作周围环境鼠侵害严重,捕获鼠类中也发现SARS CoV特异基因。
Objectives To understand the presence of SARS-CoV-specific genes in murine and murine surrounding environmental SARS cases in 2004, to explore the possibility of murine SARS as a medium or source of SARS. Methods The murine densities were measured by visual field mouse and mouse cage method and the specific SARS CoV gene was amplified by nested RT-PCR. The amplified products were sequenced and analyzed by DNASTAR software. Results The first patient lived in LJ Garden Building and the surrounding buildings were more serious in the environment of environment protection. Fifty key places such as ceilings and power distribution rooms in the ground floor were examined, with a positive rate of 26% Surroundings of the building accumulated 200m, 6 of which were found in rats; the positive rate of squirrel cage was 7.9%; RT-PCR was used to detect the lung tissue and anal Swabs, 6 of which were positive in the lung tissues of the Rattus flavipectum, one was positive for the anal swab and the rest were negative. The nucleotide sequence of the amplified product was 95% identical to the SARS gene sequence and 96%. Conclusion In 2004, the environmental SARS cases were seriously affected by the first SARS cases and SARS CoV-specific genes were also found in the captured mice.