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为给科研人员和仪器管理者提供系统、直观的激光共聚焦显微镜的使用与维护方法,介绍了Olympus倒置激光共聚焦显微镜与活细胞工作站(IX81-FV1000)的配置、软硬件组成和技术参数,样品制备要求,单光子、活细胞图像采集常用操作规程,共享成效、日常维护和管理等方面。浙江大学医学院公共技术平台的倒置激光共聚焦显微镜实行开放共享,近三年来使用率高,共享效果好。根据仪器使用经验总结出了一些获得高分辨率图像的方法,供大家探讨。
In order to provide researchers and instrument managers with a systematic and intuitive method of using and maintaining laser confocal microscopy, the configuration, software and hardware composition and technical parameters of Olympus inverted laser confocal microscope and living cell workstation (IX81-FV1000) are introduced. Sample preparation requirements, single-photon, live cell image acquisition common operating procedures, sharing effectiveness, routine maintenance and management. Zhejiang University Medical College public technology platform inverted laser confocal microscope open sharing, high utilization rate in the past three years, the sharing effect is good. According to the experience of the instrument summed up some methods to obtain high-resolution images for your discussion.