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目的:通过检测活动期ＳＬＥ患者Ｔ、Ｂ细胞ＣＤ69表达动力学,以探讨活动期ＳＬＥ体内Ｔ、Ｂ细胞活化机制。方法:ＣｏｎＡ体外刺激5例活动期ＳＬＥ患者和5例健康志愿者外周血单个核细胞,选取不同时间点,流式细胞仪观察Ｔ细胞亚群,Ｂ细胞ＣＤ69表达率。结果:ＣｏｎＡ刺激后,活动期ＳＬＥ患者ＣＤ4+、ＣＤ8+和Ｂ细胞在4ｈ后ＣＤ69表达达到高峰,健康志愿者则在24ｈ后达到高峰,随后逐渐下降,ＣＤ4+细胞比ＣＤ8细胞ＣＤ69表达要强。结论:活动期ＳＬＥ患者Ｔ、Ｂ的活化均有ＣＤ69参与,高峰出现时间较正常人提前,Ｔ、Ｂ细胞活化参与ＳＬＥ的发病过程。
Objective: To detect the expression of CD69 in T and B cells of active SLE patients in order to investigate the mechanism of T and B cell activation in active SLE patients. Methods: Peripheral blood mononuclear cells from 5 active SLE patients and 5 healthy volunteers were stimulated with ConA in vitro. The expression of CD69 in T lymphocyte subsets and B cells was observed by flow cytometry at different time points. Results: After ConA stimulation, the expression of CD69 in CD4 +, CD8 + and B cells of patients with active SLE peaked at 4h, peaked at 24h after SLE, and then decreased gradually. CD69 expression in CD4 + cells was stronger than that in CD8 cells. CONCLUSION: The activation of T and B in patients with active SLE has CD69 involvement, the peak appears earlier than normal, and the activation of T and B cells participates in the pathogenesis of SLE.