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目的检测2型糖尿病患者尿微量白蛋白(UmALB)以及血清超敏C-反应蛋白(hs-CRP)、白细胞介素-6(IL-6)和可溶性细胞黏附分子-1(sICAM-1)的水平,探讨炎症对糖尿病肾病(DN)发生、发展的影响。方法根据尿微量白蛋白排泄率(UREA)将91例2型糖尿病患者分为正常蛋白尿组30例,早期DN组29例,蛋白尿组32例;选择91例同期健康体检者作为健康对照组。分析各组炎症因子血清表达水平的差异,UmALB用速率散射比浊法测定,hs-CRP用致敏乳胶增强免疫技术测定,IL-6、sICAM-1水平的测定用酶联免疫吸附(ELISA)方法。结果 2型糖尿病组患者血清中hs-CRP[(10.63±8.31)mg/L]、IL-6[(74.35±58.94)ng/L]以及sICAM-1水平[(113.93±42.26)μg/dl]明显高于健康对照组[分别为(2.89±3.27)mg/L、(29.32±17.09)ng/L、(27.15±13.97)μg/dl],差异均有统计学意义(P<0.01),不同UREA各组之间hs-CRP、IL-6以及sICAM-1的水平差异均有统计学意义(P<0.05,P<0.01)。UmALB与hs-CRP、IL-6、sICAM-1表达量呈明显的正相关(r值分别为0.8168、0.731 8和0.8567,P<0.01)。结论炎症是DN肾病发生、发展中的重要影响因素。
Objective To detect urinary albumin (UmALB), serum hs-CRP, IL-6 and sICAM-1 in patients with type 2 diabetes mellitus Level, to explore the impact of inflammation on the occurrence and development of diabetic nephropathy (DN). Methods According to the urine microalbumin excretion rate (UREA), 91 patients with type 2 diabetes were divided into normal proteinuria group (n = 30), early DN group (n = 29) and proteinuria group (n = 32). 91 healthy people were selected as healthy control group . The expression of IL-6 and sICAM-1 in serum of each group was analyzed by enzyme-linked immunosorbent assay (ELISA) method. Results The serum levels of hs-CRP [(10.63 ± 8.31) mg / L], IL-6 [(74.35 ± 58.94) ng / L] and sICAM-1 in the patients with type 2 diabetes [(113.93 ± 42.26) μg / dl] (2.89 ± 3.27) mg / L, (29.32 ± 17.09) ng / L and (27.15 ± 13.97) μg / dl respectively), the differences were statistically significant (P <0.01) The levels of hs-CRP, IL-6 and sICAM-1 in each group of UREA were significantly different (P <0.05, P <0.01). The expression of UmALB was positively correlated with the expression of hs-CRP, IL-6 and sICAM-1 (r = 0.8168, 0.731 8 and 0.8567 respectively, P <0.01). Conclusion Inflammation is an important factor in the occurrence and development of DN nephropathy.