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以来自黑龙江省、吉林省、辽宁省、北京、山东省、江苏省、湖北省和广东省8个省份的36株黄瓜霜霉菌为试材,采用SSR分子标记的方法,研究了黄瓜霜霉病菌的遗传多样性。结果表明:中国黄瓜霜霉菌遗传分化较大,群体遗传多样性比较丰富。利用11对SSR引物共扩增出170条谱带,其中多态性条带160条,占总带数的94.1%。从11对引物中筛选出了多态性好、条带清晰的引物D13。通过NTSYS软件构建了黄瓜霜霉菌的亲缘关系树状图,聚类分析结果表明,菌株间的相似系数在0.43~1.00,说明SSR遗传多样性与菌株的地理来源存在一定的相关性。
Thirty-six cucumber downy mildew strains from eight provinces in Heilongjiang, Jilin, Liaoning, Beijing, Shandong, Jiangsu, Hubei and Guangdong provinces were used as test materials. By means of SSR molecular markers, Genetic diversity. The results showed that the Chinese cucumber downy mildew had more genetic differentiation and abundant genetic diversity. A total of 170 bands were amplified using 11 pairs of SSR primers, of which 160 bands were polymorphic, accounting for 94.1% of the total bands. Eleven pairs of primers were screened for good polymorphism and clear banding primer D13. The phylogenetic tree was constructed by NTSYS software. The cluster analysis showed that the similarity coefficient of the isolates was between 0.43 and 1.00, indicating that there was a certain correlation between SSR genetic diversity and the geographical origin of the strains.