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目的通过分析相关抗氧化酶水平并结合尿形态砷变化水平探讨砷对家兔皮肤抗氧化水平的影响。方法将30只成年健康清洁级雄性新西兰家兔随机分为5组,分别为0(对照)、0.075(1/100 LD50)、0.15(1/50 LD50)、0.375(1/20 LD50)、0.75(1/10 LD50)mg/kg,每组6只。采用自由饮水方式进行染毒,连续12周。检测家兔皮肤组织中8-羟基脱氧鸟苷(8-OHd G)、过氧化氢酶(CAT)、髓过氧化物酶(MPO)含量及血红素氧合酶1(HO-1)活力;同时,检测家兔尿中As(Ⅲ)、As(Ⅴ)、一甲基胂酸(MMA)和二甲基胂酸(DMA)含量,并分析砷代谢情况[一甲基化率(PMI)、二甲基化率(SMI)]。结果与对照组相比,0.150、0.375、0.750 mg/kg亚砷酸钠染毒组家兔皮肤组织内CAT和HO-1活力均较低,而0.750mg/kg亚砷酸钠染毒组家兔皮肤组织内8-OHd G含量较高,差异有统计学意义(P<0.05);各剂量亚砷酸钠染毒组家兔皮肤组织内MPO活力均无明显变化。与对照组相比,0.750 mg/kg亚砷酸钠染毒组家兔尿中总砷、As(Ⅲ)、DMA的含量和0.375、0.750 mg/kg亚砷酸钠染毒组家兔尿中MMA含量及各剂量亚砷酸钠染毒组PMI值以及0.150、0.375、0.750 mg/kg亚砷酸钠染毒组SMI值均较高,差异有统计学意义(P<0.05);而各剂量亚砷酸钠染毒组家兔尿中As(Ⅴ)的含量均无明显变化。结论砷暴露可能通过促进家兔体内砷代谢产物MMA含量以及PMI进而抑制相关抗氧化酶,从而降低抗氧化水平,导致机体氧化损伤。
Objective To investigate the effects of arsenic on the anti-oxidation of rabbit skin by analyzing the level of anti-oxidase and the level of arsenic in urine. Methods Thirty adult healthy and clean male New Zealand rabbits were randomly divided into five groups: 0 (control), 0.075 (1/100 LD50), 0.15 (1/50 LD50), 0.375 (1/10 LD50) mg / kg, 6 in each group. Use free drinking water for 12 weeks. The levels of 8-OHdG, CAT, MPO and HO-1 in rabbits skin were measured. At the same time, the urinary As (Ⅲ), As (Ⅴ), monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) contents in rabbits were detected and their arsenic metabolism [monomethylation rate (PMI) , Dimethylation rate (SMI)]. Results Compared with the control group, the activities of CAT and HO-1 in the skin tissue of 0.1,03,0.375,0.750 mg / kg sodium arsenite-treated rabbits were lower than those of the control group, while those of 0.750 mg / kg sodium arsenite- The content of 8-OHd G in rabbit skin tissue was higher than that in the control group (P <0.05). There was no significant change in the MPO activity in the skin tissue of rabbits treated with sodium arsenite. Compared with the control group, urinary total arsenic, As (Ⅲ) and DMA in 0.750 mg / kg sodium arsenite treatment group and urine in 0.375 and 0.750 mg / kg arsenite treatment group MMA content and each dose of sodium arsenite exposure group PMI and 0.150,0.375,0.750 mg / kg ASA exposure group SMI values were higher, the difference was statistically significant (P <0.05); while each dose There was no significant change in urinary As (Ⅴ) content in the rabbits treated with sodium arsenite. Conclusion Arsenic exposure may reduce the level of antioxidant and lead to oxidative damage by ameliorating the levels of arsenic metabolites MMA and PMI in rabbits.