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以4-肼基-7-硝基-2,1,3-苯并氧杂二唑肼为荧光底物,乙烯基羧酸酯、伯醇为转酯化反应底物,通过荧光分光光度法比较了几种能以固体聚合物为底物的商品化酶制剂在有机溶剂中催化转酯化反应的性质。该方法使用微量溶剂和底物进行反应,测定快捷,具有很高的直观性。实验所用4种酶制剂均可在异辛烷、甲苯和乙腈中催化转酯化反应,催化能力是脂肪酶LPL-3>蛋白酶Alcalase 3.0 T>蛋白酶subtilisin Carlsberg>蛋白酶bacillolysin,但有机溶剂的疏水性对转酯化反应影响较大,疏水性高的有机溶剂比较适合做酶催化转酯化反应的溶剂。和月桂酸乙烯酯相比,小分子的丙酸乙烯酯更有利于酶催化转酯化反应的进行。
Using 4-hydrazino-7-nitro-2,1,3-benzoxadiazole hydrazide as the fluorescent substrate, vinyl carboxylate and primary alcohol as the transesterification substrate, the fluorescence spectrophotometry The properties of several commercial enzyme preparations that use solid polymer as substrate for catalytic transesterification in organic solvents are compared. The method uses a trace amount of solvent and substrate reaction, the determination of fast, highly intuitive. The four enzyme preparations used in the experiment catalyzed the transesterification reaction in isooctane, toluene and acetonitrile. The catalytic ability was lipase LPL-3> Alcalase 3.0 T> protein subtilisin Carlsberg> protease bacillolysin, but the hydrophobicity of organic solvents On the transesterification reaction greater hydrophobic organic solvent is more suitable for enzyme-catalyzed transesterification solvent. Compared to vinyl laurate, small molecules of vinyl propionate are more favorable for the enzymatic transesterification.