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目的:观察健脾益气明目胶囊对N-甲基-N-亚硝脲(MNU)诱导的SD大鼠视网膜变性的保护作用。方法:出生后43d的雌性SD大鼠30只随机分为3组(n=10):正常对照组、模型对照组、药物组。药物组以健脾益气明目胶囊溶解后灌胃,正常组和模型组以等量纯化水灌胃,bid,连续7d。于生后50d,药物组和模型组大鼠腹腔注射MNU40mg.kg-1,正常组皮下注射等量生理盐水做对照。观察MNU处理后12,24,48,72h各组大鼠视网膜电图中a波及b波的变化。结果:正常对照组大鼠不同时间点视网膜电图(ERG)a波及b波的振幅差异无显著性,模型对照组在MNU处理后ERGa波及b波的振幅均呈持续性、进行性下降,健脾益气明目胶囊可明显抑制ERGa波及b波振幅的降低。结论:健脾益气明目胶囊对MNU诱导的大鼠视网膜变性有保护作用,是治疗视网膜变性性疾病的有效中药复方制剂。
Objective: To observe the protective effect of Jianpi Yiqi Mingmu capsule on retinal degeneration induced by N-methyl-N-nitrosourea (MNU) in SD rats. Methods: Thirty female SD rats born at 43 days after birth were randomly divided into 3 groups (n = 10): normal control group, model control group and drug group. The drug group was given spleen and qi Mingmu Capsule dissolved, gavage, normal group and model group with the same amount of purified water gavage bid for 7d. At 50 days after birth, the rats in the drug group and the model group were intraperitoneally injected with MNU 40 mg.kg-1, and the rats in the normal group were injected with the same amount of normal saline as the control. The changes of a wave and b wave in the electroretinograms of rats in each group were observed after MNU treatment for 12, 24, 48 and 72 hours. Results: There was no significant difference in the amplitudes of ERG a and b wave in normal control rats at different time points. The amplitudes of ERGa and b wave in MNU group were both persistent and progressive Spleen Yiqi Mingmu capsules can significantly inhibit ERGa waves and b-wave amplitude reduction. Conclusion: Jianpi Yiqi Mingmu Capsule has a protective effect on retinal degeneration induced by MNU in rats. It is an effective traditional Chinese medicine compound preparation for the treatment of retinal degenerative diseases.