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目的 :检测黑色素瘤抗原基因 1、 3(MAGE 1和MAGE 3)在胃癌中的表达 ,探索其在胃癌免疫治疗中的应用价值。方法 :采用RT PCR法 ,对 36例胃癌患者的癌组织和相应的癌旁“正常”黏膜 ,以及 37例慢性胃炎患者的胃镜活检组织中 ,MAGE 1和MAGE 3基因的表达进行研究 ,并对RT PCR扩增产物中的目的基因片段进行DNA测序验证。结果 :36例胃癌组织中 ,MAGE 1基因的表达阳性率为 72 .2 2 % (2 6 / 36 ) ,MAGE 3基因的表达阳性率为 6 9.4 4 % (2 5 / 36 )。MAGE 1、MAGE 3的表达均为阳性者为 5 2 .78% (19/ 36 )。癌旁“正常”黏膜中 ,MAGE 1、MAGE 3基因的表达阳性率分别为 4 7.2 2 % (17/ 36 )和4 4 .4 4 % (16 / 36 ) ;MAGE 1、MAGE 3基因表达双阳性者为 30 .5 5 % (11/ 36 )。在 37例胃慢性炎症患者中 ,MAGE 1、MAGE 3基因的表达阳性率 ,分别为 2 9.73% (11/ 37)和 2 7.0 3% (10 /37) ;MAGE 1、MAGE 3基因的表达双阳性者为 8.1% (3/ 37)。胃癌组织中 ,MAGE 1和MAGE 3基因表达阳性率均高于癌旁和胃慢性炎症患者 ;在肿瘤组织中的表达阳性率与肿瘤浸润的深度和分化程度有关 (P <0 .0 5 ) ;而与临床病理分期及淋巴结转移无关 (P >0 .0 5 )。结论 :基于MAGE 1和MAGE 3基因在胃癌中的高表达率 ,可利用这两种蛋白作为靶?
Objective: To detect the expression of melanoma antigen genes 1 and 3 (MAGE 1 and MAGE 3) in gastric cancer, and to explore its value in the immunotherapy of gastric cancer. Methods: The expression of MAGE 1 and MAGE 3 genes in 36 cases of gastric cancer tissues, corresponding “normal” mucosa and 37 cases of chronic gastritis biopsies were studied by RT-PCR. RT PCR amplification of the target gene fragment for DNA sequencing validation. Results: The positive rate of MAGE 1 gene was 72.22% (26/36) in 36 gastric cancer tissues, and 6 9.44% (2.5 / 36) in MAGE 3 gene. The positive expression of MAGE 1 and MAGE 3 was 52.78% (19/36). The positive expression rates of MAGE 1 and MAGE 3 gene in the adjacent normal mucosa were 4 7.22% (17/36) and 44.44% (16/36), respectively. MAGE 1 and MAGE 3 genes expressed double positive The number of sexes was 30.55% (11/36). The positive expression rates of MAGE 1 and MAGE 3 in 37 patients with gastric chronic inflammation were 2 9.73% (11/37) and 2 7.03% (10/37), respectively. The positive expression rates of MAGE 1 and MAGE 3 genes Sex was 8.1% (3/37). The positive rates of MAGE 1 and MAGE 3 gene expression in gastric cancer tissues were higher than those in paracancerous and chronic gastritis patients. The positive rates of MAGE 1 and MAGE 3 expression were correlated with the depth and differentiation of tumor infiltration (P <0.05). But not with clinicopathologic stage and lymph node metastasis (P> 0.05). Conclusion: Based on the high expression of MAGE 1 and MAGE 3 in gastric cancer, can these two proteins be used as targets?