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目的探讨c-myc致癌蛋白和细胞增殖因子ki-67与葡萄膜恶性黑色素瘤临床组织病理特性的关系。方法采用免疫组织化学法检测40例葡萄膜恶性黑色素瘤石蜡包埋标本中c-myc致癌蛋白和Ki-67蛋白的表达。结果 40例葡萄膜恶性黑色素瘤标本中,c-myc在23例肿瘤的细胞胞浆或胞核中表达,阳性率为57.5%。c-myc的阳性表达与肿瘤的大小及患者的年龄有关(P<0.05),而与肿瘤的细胞类型、肿瘤的位置、巩膜浸润程度和性别无关(P>0.05)。Ki-67指数的表达水平与肿瘤细胞分型(特别是上皮细胞型)和肿瘤的位置有关(P<0.05)。未发现Ki-67表达与肿瘤的大小、巩膜浸润程度及年龄、性别有关。且c-myc与Ki-67指数呈正相关(r=0.654,P<0.05)。结论 c-myc和Ki-67在葡萄膜恶性黑色素瘤中的表达是影响预后的因素,可以作为临床上判断预后和指导治疗的重要指标。
Objective To investigate the relationship between c-myc oncogene protein and cell proliferation factor ki-67 and histopathological features of uveal melanoma. Methods Immunohistochemistry was used to detect the expression of c-myc oncogene and Ki-67 protein in 40 cases of uveal melanoma paraffin-embedded specimens. Results 40 cases of uveal malignant melanoma specimens, c-myc in 23 cases of tumor cell cytoplasm or nucleus, the positive rate was 57.5%. The positive expression of c-myc was related to the size of the tumor and the age of the patient (P <0.05), but not to the cell type, the location of the tumor, the degree of scleral infiltration and gender (P> 0.05). The expression of Ki-67 index was correlated with tumor cell type (especially epithelial cell type) and tumor location (P <0.05). Ki-67 expression was not found and the size of the tumor, scleral infiltration and age, gender-related. There was a positive correlation between c-myc and Ki-67 index (r = 0.654, P <0.05). Conclusion The expression of c-myc and Ki-67 in uveal melanoma is a prognostic factor that may be used as an important index to judge the prognosis and guide the treatment.