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DNA甲基化对于植物的生长发育和组织分化具有十分重要的调控作用.用MSAP(methylation-sensitiveamplifiedpolymorphism)和HPLC两种方法分析了油菜种子萌发过程中DNA甲基化的动态变化过程,并且比较了不同器官组织的甲基化水平差异.MSAP的分析结果表明,油菜种子基因组中大约有15.7%的CCGG位点发生了胞嘧啶甲基化,发生甲基化的方式以C5mCGG双链甲基化为主;种子萌发过程中同时发生甲基化和去甲基化事件,其中去甲基化占据主导地位;不同器官组织的甲基化水平存在一定差异,胚根的甲基化水平最低,下胚轴次之,子叶最高,HPLC分析结果与此一致.最后,对11个甲基化多态性片段进行了序列分析,发现基因编码区和非编码区发生DNA甲基化的频率基本相等.由此可见,种子萌发过程中DNA的甲基化变化是一个十分复杂的过程,油菜可能通过甲基化和去甲基化的方式调控基因的表达,并最终决定植株的生长发育和器官分化.
DNA methylation plays an important regulatory role in the growth and differentiation of plants.Dynamic changes of DNA methylation in rape seeds during germination are analyzed by MSAP (methylation-sensitiveamplifiedpolymorphism) and HPLC Differences in methylation levels of different organs and tissues.MSAP analysis showed that approximately 15.7% of the CCGG locus in the rapeseed genome was cytosine methylated, methylation occurred in C5mCGG double-stranded methylation In the course of seed germination, both methylation and demethylation events occurred simultaneously, of which demethylation dominated. The methylation level of different organ tissues was different, and the radical methylation level was the lowest. The highest frequency of the cotyledons and the same result of HPLC analysis.Finally, 11 methylated polymorphic fragments were sequenced and found that the frequency of DNA methylation in gene coding region and non-coding region was basically the same. It can be seen that DNA methylation changes during seed germination is a very complicated process. Rape may regulate gene expression through methylation and demethylation, and ultimately Plant growth and organ differentiation.