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采用改进的分散聚合方法制得的纳米级聚苯乙烯微球(PS)为种球,以罗丹明B为模板分子,通过单步溶胀聚合法在水相中制备了单分散分子印迹聚合物微球(MIPs)。通过红外光谱分析MIPs的结合位点,利用紫外光谱研究MIPs的结合机理和识别特性,并用扫描电子显微镜观察其形貌,采用荧光光度法,研究荧光分子印迹聚合物的吸附结合能力及荧光特性。结果表明:PS微球的粒径分布100~150nm,MIPs粒径分布为200~300nm,罗丹明B分子印迹聚合物对罗丹明B具有特异的识别选择性和优良的结合特性,荧光光谱法检测MIPs在557nm激发波长下发射波长为593nm,荧光较强。
Nanosized polystyrene microspheres (PS) prepared by the improved dispersion polymerization method were bulbs. Rhodamine B was used as template to prepare monodisperse molecularly imprinted polymers Balls (MIPs). The binding sites of MIPs were analyzed by infrared spectroscopy. The binding mechanism and recognition properties of MIPs were studied by ultraviolet spectroscopy. The morphology of MIPs was observed by scanning electron microscopy. The binding ability and fluorescence properties of MIPs were studied by fluorescence spectrophotometry. The results showed that the particle size distribution of PS microspheres was 100-150 nm and the size distribution of MIPs was 200-300 nm. Rhodamine B molecularly imprinted polymer had specific recognition selectivity and excellent binding property to Rhodamine B, and its fluorescence spectrum MIPs at 557nm excitation wavelength emission wavelength of 593nm, strong fluorescence.