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研究了门多萨假单胞菌ＤＲ８（ＰｓｅｕｄｏｍｏｎａｓｍｅｎｄｏｃｉｎａＤＲ８）菌株对单甲脒农药的降解代谢．该菌利用单甲脒作为生长的唯一氮源．其对单甲脒的呼吸作用试验确证了该农药的生物可降解性．单甲脒对细菌生物氧化代谢关联酶活性的影响研究表明，脱氢酶对单甲脒较敏感，而ＮＡＤＨ氧化酶则耐受性较强．单甲脒生物降解特性研究表明，ＤＲ８菌株的单甲脒降解酶为组成酶，主要分布于细胞壁和细胞膜组分，该酶作用于单甲脒的Ｋｍ值约为５１４ｍｍｏｌ／Ｌ．经紫外吸收光谱和薄层层析等分析检测，单甲脒降解过程中形成氨和２，４二甲基苯胺．该菌对于单甲脒农药的降解属于共代谢作用．
The degradation and metabolism of monoform amidine pesticides were studied in Pseudomonas mendocina DR-8 strain. The strain utilizes monoamidine as the sole nitrogen source for growth. Respiratory testing of monoamidine confirmed the biodegradability of the pesticide. The effect of monoamidine on the activity of bacterially oxidative and metabolic enzymes was studied. The results showed that dehydrogenase was more sensitive to monoamidine and NADH oxidase was more tolerant. Monomethyl formamidine biodegradation studies showed that DR 8 strains of mono-formamidine degrading enzyme is a constitutive enzyme, mainly in the cell wall and cell membrane components, the enzyme acting on the single-formamidine Km value of about 5 14mmol / L. After UV absorption spectroscopy and thin layer chromatography analysis of detection, the formation of ammonia and 2,4-dimethylaniline during the degradation of monoamidine. The bacteria for the degradation of monoamidine pesticides belong to the role of co-metabolism.