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为了探讨肿瘤化疗机理，作者采用流式细胞术单参数分析方法，检测了４５例恶性肿瘤患者化疗前后外周血细胞的凋亡（Ａｐｏ）、Ｓ期细胞比率（ＳＰＦ）和增殖指数（ＰＩ）的变化。结果表明，肿瘤患者血细胞Ａｐｏ水平明显高于正常对照（Ｐ＜０．０１），化疗结束时进一步升高（Ｐ＜０．０１），化疗后１个月又恢复到化疗前的水平。肿瘤患者的ＳＰＦ和ＰＩ均显著高于正常对照（Ｐ＜０．０１），化疗结束时明显减低，ＰＩ尤为显著（Ｐ＜０．０１），并持续到化疗后１个月。从而可见，化疗使患者血细胞Ａｐｏ呈“一过性”增高，肿瘤的化疗机制主要是化疗药物使细胞增殖活性减低，细胞聚积在Ｇｏ／Ｇ１期，同时药物诱导细胞凋亡增加，达到治疗肿瘤的目的
In order to investigate the mechanism of tumor chemotherapy, the authors used flow cytometry single-parameter analysis to detect changes in peripheral blood cell apoptosis (Apo), S-phase cell ratio (SPF), and proliferation index (PI) before and after chemotherapy in 45 patients with malignant tumors. . The results showed that the level of Apo in blood cells of tumor patients was significantly higher than that of normal controls (P<0.01), further increased at the end of chemotherapy (P<0.01), and returned to the level before chemotherapy 1 month after chemotherapy. The SPF and PI of tumor patients were significantly higher than those of normal controls (P<0.01), significantly decreased at the end of chemotherapy, PI was particularly significant (P<0.01), and continued until 1 month after chemotherapy. Thus, it can be seen that chemotherapy causes Apo of blood cells to increase “transiently”. The chemotherapy mechanism of tumors is mainly due to the decrease of cell proliferation activity by chemotherapy drugs, accumulation of cells in the Go/G1 phase, and drug-induced increase in apoptosis to achieve tumor treatment. purpose