论文部分内容阅读
本文研究了NAG微量酶反应比色法在IL-2活性检测中的应用,结果表明IL-2活性测定所用的CTLL-2细胞浓度与OD值呈直线关系(r=0.964715)。本文还探讨了NAG法在IL-2活性测定中CTLL-2细胞浓度,培养时间对检测效果的影响。本法与~3H—TdR掺入法和MTT法相比有较好的一致性。
In this paper, the application of NAG micro-enzyme reaction colorimetric assay in the detection of IL-2 activity showed that the concentration of CTLL-2 cells used in the determination of IL-2 activity had a linear relationship with the OD value (r = 0.964715). This article also discussed the NAG method in the determination of IL-2 activity CTLL-2 cell concentration, culture time on the detection effect. This method and ~ 3H-TdR incorporation method and MTT method has better consistency.