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目的探讨中药溪黄草水提取物对醋氨酚所致小鼠急性肝损伤的保护作用。方法 NIH小鼠随机分为6组,其中溪黄草(低、中、高剂量)/醋氨酚组分别灌服溪黄草水提物200、400、800 mg·kg-1,溪黄草对照组灌服溪黄草提取物400 mg·kg-1,正常对照组和醋氨酚模型组灌服相同体积的生理盐水,每次间隔12h,连续7d。末次给药后1h,醋氨酚造模组和溪黄草/醋氨酚组腹腔注射给予醋氨酚(350 mg·kg-1)造急性肝损伤小鼠模型,正常对照组、溪黄草对照组则腹腔注射相同体积的生理盐水,36h后处死小鼠,收集血液和肝脏组织,检测血清ALT、AST活性和SOD、H2O2、MDA水平。结果实验造模成功,与造模组比较,灌胃给予高、中、低不同剂量的溪黄草水提物,均能显著降低小鼠血清ALT、AST活性,升高肝组织中SOD水平,清除肝脏匀浆H2O2和降低肝脏MDA含量,且该水提物对不同指标的影响均呈现明显的量效关系。结论溪黄草水提物对醋氨酚所致的小鼠急性肝损伤具有保护作用,抗氧化作用可能是其保护作用的机制之一。
Objective To investigate the protective effect of Xihuangcao water extract on acute liver injury induced by acetaminophen in mice. Methods NIH mice were randomly divided into 6 groups. The Xihuangcao (low, medium and high dose) / acetaminophen groups were given 200,400,800 mg · kg-1 of water extracts of Xihuang Cao, The rats were fed with 400 mg · kg-1 of Xihuangcao extract, and the normal control group and the model group of acetaminophen were fed with the same volume of saline at intervals of 12 hours for 7 days. At 1 hour after the last administration, acetaminophen model group and Xihuangcao / acetaminophen group were given intraperitoneal injection of acetaminophen (350 mg · kg -1) to establish acute liver injury model in mice. The normal control group, The control group received intraperitoneal injection of the same volume of normal saline. After 36 hours, the mice were sacrificed and the blood and liver tissues were collected for detection of serum ALT and AST activities and the levels of SOD, H2O2 and MDA. Results The model was successfully established. Compared with model group, high, medium and low doses of Xihuangcao aqueous extract could significantly reduce the activity of ALT and AST, raise the level of SOD in liver and clear the liver H 2 O 2 was homogenized and MDA content in the liver was decreased, and the effects of the water extract on different indexes showed significant dose-response relationship. Conclusion Water extract of Xihuangcao can protect against acute liver injury induced by acetaminophen in mice. Antioxidant effect may be one of its protective mechanisms.