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本文应用免疫荧光法检测了294例鼻咽癌、118例鼻咽粘膜病变和40例正常人血清的VCA-IgA、IgG,EA-IgA,IgG。89.8％和26.7％鼻咽癌患者为VCA-IgA和EA-IgA抗体阳性,抗体的几何平均滴度为15.21和1.9。只有7.5％正常人和15.3％鼻咽粘膜病变者有低滴度的VCA-IgA抗体,而EA-IgA抗体,正常人全部阴性,粘膜病变者有2.5％阳性(GMT为1.30)。这些结果表明IgA抗体的出现是鼻咽癌EA病毒血清学的非常特异的现象,其中EA-IgA的阳性率和GMT均较低,但具有更高的特异性。所有被检对象的血清均为VCA-IgG阳性,但鼻咽癌组抗体的GMT(107.67)显著地高于正常人组(64.98)和粘膜病变组(26.20)。EA-IgG在各组中亦有显著差异,提示抗EA抗体也是鼻咽癌的特异现象。 鼻咽癌患者晚期EBV抗体水平显著高于早期。但抗体水平与原发灶大小无关,与淋巴结转移程度有关。在鼻咽癌患者体内,IgA和IgG抗体水平的升高是一致的。 本文还比较了免疫荧光法和免疫酶法在测定VCA-IgA的结果。免疫酶法检出抗体的阳性率和GMT稍高于前者,但无统计学上的差异,两法的特异性也是一致的。
In this paper, immunofluorescence method was used to detect VEGF, VCA-IgA, IgG, EA-IgA, IgG in 294 cases of nasopharyngeal carcinoma, 118 cases of nasopharyngeal mucosal lesions and 40 cases of normal human serum. 89.8% and 26.7% of NPC patients were positive for VCA-IgA and EA-IgA antibodies, and the geometric mean antibody titers were 15.21 and 1.9. Only 7.5% of normal persons and 15.3% of nasopharyngeal mucosa lesions had low-titered VCA-IgA antibodies, whereas EA-IgA antibodies were negative in normal individuals and mucosal lesions were 2.5% positive (GMT 1.30). These results indicate that the presence of IgA antibodies is a very specific phenomenon in the serology of nasopharyngeal carcinoma EA virus, in which the positive rate and GMT of EA-IgA are lower, but with higher specificity. The serum of all subjects was positive for VCA-IgG, but the GMT (107.67) of the antibody in the nasopharyngeal carcinoma group was significantly higher than that of the normal (64.98) and mucosal lesions (26.20). EA-IgG was also significantly different in each group, suggesting that anti-EA antibody is also a specific phenomenon of nasopharyngeal carcinoma. Advanced EBV antibody levels were significantly higher in patients with nasopharyngeal carcinoma than in early stages. However, the antibody level was not related to the size of the primary tumor and was related to the degree of lymph node metastasis. In patients with nasopharyngeal carcinoma, the elevated levels of IgA and IgG antibodies are consistent. This article also compared the results of immunofluorescence and immunoenzymatic assays for the determination of VCA-IgA. The positive rate of antibody detection by immunoenzymatic assay and GMT was slightly higher than the former, but there was no statistical difference. The specificity of the two methods was also consistent.