近二十多年来,细胞融合已成为一项主要的细胞工程技术。寻找更理想的诱导剂和诱导方法,一直是细胞融合研究的中心问题之一。近年来已经证明可以应用电场诱导的方法对差异较大的植物细胞和动物细胞进行融合,并能产生具有新的形质,有生活力的杂种细胞。1980年西德的 U.Zimmerman 首先报告了电场诱导细胞融合的新技术。简单地说,这种技术具有明显的优点,细胞在电场中极化成偶极子,并 In the past two decades, cell fusion has become a major cell engineering technology. The search for more desirable inducers and induction methods has been one of the central issues in cell fusion studies. In recent years, it has been demonstrated that electric field induced methods can be used to fuse plant cells and animal cells that are different from each other and produce hybrid cells with new qualities and viability. In 1980 West U.Zimmerman first reported a new technique of electric field-induced cell fusion. Simply put, this technique has the obvious advantage that cells are polarized into dipoles in an electric field, and