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目的了解＋ＧＺ重复暴露后大鼠脑组织神经生长因子（ＮＧＦ）和碱性成纤维细胞生长因子（ｂＦＧＦ）基因表达的变化，探讨两种生长因子在＋ＧＺ暴露所致脑损伤中的作用和意义。方法２４只雄性Ｗｉｓｔａｒ大鼠随机分成对照组、＋ＧＺ重复暴露后３０ｍｉｎ、６ｈ和２４ｈ四组，每组６只。对照组大鼠Ｇ值为＋１ＧＺ，实验组大鼠在动物离心机上经历了３次＋１０ＧＺ／３ｍｉｎ（两次间间隔３０ｍｉｎ）作用。分别于暴露后３０ｍｉｎ、６ｈ和２４ｈ处死大鼠取脑，提取总ＲＮＡ，用半定量反转录聚合酶链反应（ＲＴ－ＰＣＲ）检测方法检测＋ＧＺ重复暴露后大鼠脑组织ＮＧＦ和ｂＦＧＦｍＲＮＡ表达水平。结果＋ＧＺ重复暴露后３０ｍｉｎ和６ｈ，大鼠脑组织ＮＧＦ和ｂＦＧＦｍＲＮＡ明显升高，２４ｈ降至正常。结论＋ＧＺ重复暴露可诱导大鼠脑组织ＮＧＦ和ｂＦＧＦｍＲＮＡ的表达增加，这两种生长因子的表达增加可能对神经细胞具有保护和损伤修复作用，是脑的自身保护机制之一。
Objective To investigate the changes of gene expression of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) in rat brain after + GZ repeated exposure and explore the roles and significance of the two growth factors in brain injury induced by + GZ exposure. Methods Twenty-four male Wistar rats were randomly divided into control group, GZ + GZ group after repeated exposure for 30min, 6h and 24h. The G value of the control group was + 1GZ, and the rats in the experimental group underwent 3 times + 10GZ / 3min in the animal centrifuge (the interval was 30min between two times). The rats were sacrificed at 30 min, 6 h and 24 h after exposure, respectively. The total RNA was extracted and the expression of NGF and bFGF mRNA in the rat brain after + GZ repeated exposure was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) . Results At 30 min and 6 h after repeated exposure of GZ, the NGF and bFGF mRNA in brain tissue of rats were significantly increased and decreased to normal at 24 h. Conclusions + GZ repeated exposure can induce the expression of NGF and bFGF mRNA in rat brain increased. The increased expression of these two growth factors may have protective and injury repair effects on nerve cells, which is one of the mechanisms of brain self-protection.