应用于癌和可遗传的传染性疾病的分子生物学研究,大多是通过分析从靶组织和血标本提取的高分子量DNA。采用无离子去污剂和有机溶剂破碎细胞膜和去除蛋白,经离心浓缩血细胞,然后消化细胞,所需血液量很少,故采用NaI溶解全血,移出含DNA的透明层,用三氯甲烷:异戊醇混合物除去血红蛋白和其他变性蛋白以提取DNA,每毫升血液可提得40～50μgDNA。以HIV-1感染和正常健康人血液试验,观察HIV-1 DNA序列的存在情况,对NaI法提取的DNA应用HIV-lgag基因特异的启动 Molecular biology studies applied to cancerous and heritable infectious diseases are mostly performed by analyzing high molecular weight DNA extracted from target tissues and blood samples. Using ion-free detergents and organic solvents to break up the cell membrane and remove the protein, concentrating the blood cells by centrifugation and then digesting the cells, the amount of blood required is very small, so whole blood is solubilized with NaI, the clear layer containing DNA is removed, Isoamyl alcohol mixtures remove hemoglobin and other denatured proteins to extract DNA, yielding 40-50 μg of DNA per ml of blood. To HIV-1 infection and normal healthy blood test to observe the presence of HIV-1 DNA sequence, the DNA extracted by NaI method using HIV-lgag gene-specific start