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采用改进的平板培养法在国内首先建立了铜绿假单胞菌生物被膜（Ｂｉｏｆｉｌｍ，ＢＦ），利用银染法检测铜绿假单胞ＢＦ的存在，通过扫描电镜观察了它的形态结构，利用计算机图像处理分析系统对经不同浓度盐酸氟罗沙星作用后银染的铜绿假单胞菌ＢＦ进行了分析统计。结果表明，平均灰度和透光率均随着浓度的升高而呈上升趋势，组间差异非常显著（Ｐ＜０．００１），提示盐酸氟罗沙星对铜绿假单胞菌ＢＦ的抑制作用在一定抗菌药物浓度范围内随着抗菌药物浓度的升高而加强。此结果与电镜和银染后光镜观察结果相同。本文旨在为临床治疗细菌生物被膜相关感染提供翔实可靠的实验依据。
Biofilm (BF) was firstly established in China by improved plate culture method. The existence of BF was detected by silver staining. The morphological structure of Pseudomonas aeruginosa BF was observed by scanning electron microscopy. Analysis of the system by the different concentrations of fleroxacin hydrochloride silver stain of Pseudomonas aeruginosa BF were analyzed. The results showed that both the average gray and the light transmittance increased with the increase of the concentration, the difference between the groups was significant (P <0.001), suggesting that the inhibitory effect of fleroxacin hydrochloride on P. aeruginosa BF Certain antibacterial drug concentration range with the concentration of antimicrobial drugs increased. The results with the electron microscope and silver staining after the same observation. This article aims to provide accurate and reliable experimental evidence for the clinical treatment of bacterial biofilm-related infections.