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于油菜初花期与盛花期采取油菜不同部位花瓣和花药进行真菌分离.除菌核病菌(Sclerotinia sclerotiorum)外,还以不同的频率分离到葡萄孢菌(Botrytis spp.),交链孢菌(Alternaria spp.),芽枝霉菌(Cladosporium spp.),叶点霉菌(Phyllosticta sp.),附球孢菌(Epicoccum sp.),镰刀菌(Fusarium spp.).其中葡萄孢菌、交链孢菌、芽枝霉菌出现较早,为初花期主要真菌群;盛花期还分离到叶点霉菌和附球孢菌,以镰刀菌出现最迟.1991年从分离真菌中选择101个菌株.通过培养皿法和花瓣法测定对菌核菌的拮抗作用,初步筛选出A_3,A_(25),A_(26),B_(10),B_(13),B_(11),B_(26),B_(31),B_(3(?)),C_8,E_1和E_2 12个对菌核菌拮抗作用较强的菌株.用以上拮抗菌和菌核菌的孢子悬液于24h 时内在实验室条件下先后喷雾接种开花的油菜植株,所有拮抗菌占领花朵百分率为63%～96%不等,而菌核菌不能定殖其上.1992年从151个分离菌株中,筛选出59个对核盘菌具有拮抗作用的真菌菌株,其中葡萄孢菌41个占69.49%,交链孢菌5个占8.47%,芽枝霉菌1个占1.69%,镰刀菌12个占20.34%.
At the early flowering stage and flowering stage of rapeseed, the petals and anthers of different parts of rape were used to isolate the fungi.These isolates of Sclerotinia sclerotiorum were also isolated at different frequencies from Botrytis spp., Alternaria spp., Cladosporium spp., Phyllosticta sp., Epicoccum sp., Fusarium spp., of which Botrytis, Alternaria, Budding mold appeared earlier, which was the primary fungus group at the beginning of florescence.Fusariums and coccus spores were also isolated from the full-flowering stage, with Fusarium occurring at the latest.191 strains were isolated from the isolated fungi in 1991. The petri dishes (26), B_ (10), B_ (13), B_ (11), B_ (26), B_ (26) and A_3 31), B_ (3 (?)), C_8, E_1, and E_2, respectively.Their spore suspensions of the above antagonistic bacteria and sclerotinia were cultured under laboratory conditions Spraying the flowering rapeseed plants, all the antagonistic bacteria occupy the percentage of flowers ranging from 63% to 96%, and sclerotinia can not colonize them.In 1992 from 151 isolates, screened 59 pairs Sclerotiorum fungal strain having antagonistic activity, wherein the Botrytis 41 accounted for 69.49%, Alternaria 5 accounted for 8.47%, Cladosporium mold 1 1.69% 12 Fusarium accounting 20.34%.